Expression profile of Nanos2 gene in dairy goat and its inhibitory effect on Stra8 during meiosis. (October 2014)

• Record Type: Journal Article
• Authors: Yao, X.; Tang, F.; Yu, M.; Zhu, H.; Chu, Z.; Li, M.; Liu, W.; Hua, J.; Peng, S.
• Is Part Of: Cell proliferation. Volume 47:Number 5(2014:Oct.)
• Page Start: 396
• Page End: 405
• Publication Date: 2014-10
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12128
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12128-abs-0001"> <title>Abstract</title> <sec id="cpr12128-sec-0001" sec-type="section"> <title>Objectives</title> <p>Nanos2, an RNA‐binding protein, belongs to the <italic>Nanos</italic> gene‐coding family and contains two CCHC zinc‐finger motifs. In mouse, it plays a pivotal role in male germ cell development, and self‐renewal of spermatogonial stem cells. However, little is known of its expression pattern and functions in dairy goat testis.</p> </sec> <sec id="cpr12128-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Immunohistochemistry and quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) were used to generate the expression profile of <italic>Nanos2</italic> in dairy goat testis. Furthermore, its overexpression effects on male germline stem cells (mGSCs) were studied using qRT‐PCR, immunofluorescence, dual‐luciferase reporter assay and western blotting.</p> </sec> <sec id="cpr12128-sec-0003" sec-type="section"> <title>Results</title> <p> <italic>Nanos2</italic> is a conservative gene expressed widely in various tissues, especially in pancreas, and it displays higher expression in adult testes than in other age groups. Overexpression of <italic>Nanos2</italic> significantly downregulated meiosis‐related genes, including <italic>Stra8</italic> and <italic>Scp3</italic>, which induced inhibition of meiosis. Results from dual‐luciferase reporter assay and western blotting indicated that <italic>Nanos2</italic> directly downregulated <italic>Stra8</italic> in goat GmGSCs.</p> </sec> <sec id="cpr12128-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Taken together, these results suggest that <italic>Nanos2</italic> plays an important role in spermatogonia and that its overexpression restrained meiosis in the dairy goat.</p> </sec> </abstract>