Immunomodulatory effect of melatonin in SK‐LU‐1 human lung adenocarcinoma cells co‐cultured with peripheral blood mononuclear cells. (23rd July 2014)

• Record Type: Journal Article
• Authors: Plaimee, P.; Khamphio, M.; Weerapreeyakul, N.; Barusrux, S.; Johns, N. P.
• Is Part Of: Cell proliferation. Volume 47:Number 5(2014:Oct.)
• Page Start: 406
• Page End: 415
• Publication Date: 2014-07-23
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12119
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12119-abs-0001"> <title>Abstract</title> <sec id="cpr12119-sec-0001" sec-type="section"> <title>Objectives</title> <p>The anti‐cancer potential of melatonin has been examined using a variety of experimental approaches. Melatonin immunomodulatory action was evaluated against the lung cancer cell line SK‐LU‐1, in co‐culture with human peripheral blood mononuclear cells (PBMC).</p> </sec> <sec id="cpr12119-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Melatonin was tested on the cell line only after 24 h incubation (direct effect), and on the co‐culture system of SK‐LU‐1 and PBMC to investigate any indirect effect. Apoptotic induction of the cancer cells was assessed using annexin V/PI staining with flow cytometric analysis for membrane alteration. Intracellular superoxide anion (O<sub>2</sub><sup><bold>−</bold></sup>) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) for intracellular oxidative stress and glutathione (GSH) for intracellular anti‐oxidation were measured with specific fluorescence probes. DNA fractions were measured employing propidium iodide (PI) fluorescence staining.</p> </sec> <sec id="cpr12119-sec-0003" sec-type="section"> <title>Results</title> <p>High doses of melatonin were directly toxic to SK‐LU‐1 cells, while PBMC‐mediated indirect effect occurred after moderate doses (1 μ<sc>m</sc>). Under co‐culture conditions, increases in apoptotic cell death, increase in oxidative stress by reduction of GSH and cell cycle arrest in G<sub>0</sub>/G<sub>1</sub> in SK‐LU‐1 cells, were observed as the immunomodulatory effect of melatonin.</p> </sec> <sec id="cpr12119-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Melatonin had indirect effects on lung cancer cells by enhancement of immunomodulatory effects, but further studies of mechanism(s) involved are needed.</p> </sec> </abstract>