Effect of dietary chitosan on growth performance, haematology, immune response, intestine morphology, intestine microbiota and disease resistance in gibel carp (Carassius auratus gibelio). (20th February 2014)

• Record Type: Journal Article
• Authors: Chen, Y.; Zhu, X.; Yang, Y.; Han, D.; Jin, J.; Xie, S.
• Is Part Of: Aquaculture nutrition. Volume 20:Number 5(2014:Oct.)
• Page Start: 532
• Page End: 546
• Publication Date: 2014-02-20
• Subjects: Aquaculture -- Periodicals; Aquatic animals -- Feeding and feeds -- Periodicals; Fishes -- Feeding and feeds -- Periodicals; 639.3
• DOI: 10.1111/anu.12106
• Languages: English
• ISSNs: 1353-5773

Abstract

<abstract abstract-type="main" id="anu12106-abs-0001"> <title>Abstract</title> <p>A 75‐day experiment was conducted with juvenile gibel carp (<italic>Carassius auratus gibelio</italic>) (4.80 ± 0.01 g) to evaluate effects of dietary chitosan on fish growth performance, haematology, intestine morphology and immune response. Six isonitrogenous (crude protein: 383 g kg<sup>−1</sup>), isolipid (97.5 g kg<sup>−1</sup>) and isocaloric (gross energy: 16.7 kJ g<sup>−1</sup>) diets were formulated to contain 0, 1800, 4000, 7500, 10 000, 20 000 mg kg<sup>−1</sup> chitosan, respectively. The results showed that the growth was depressed when the fish fed with 10 000 mg kg<sup>−1</sup> chitosan. Serum cholesterol, triglyceride and low‐density lipoprotein decreased in 10 000 and 20 000 mg kg<sup>−1</sup> chitosan. On day 75, blood leucocyte phagocytic activity respiratory burst and alternative pathway of complement haemolytic activity were enhanced in 4000 mg kg<sup>−1</sup> chitosan. The number of goblet cell, intraepithelial lymphocyte of mid‐intestine and microvilli height of distal intestine increased at 4000 mg kg<sup>−1</sup> dietary chitosan. Dietary chitosan modulated intestine microbiota, depressed pathogen bacteria <italic>Aeromonas veronii‐</italic>like and improved <italic>Cellulomonas hominis</italic>‐like, <italic>Bacillus oceanisediminis</italic>‐like and two uncultured bacterium‐like species on day 75. Dietary 7500 and 10 000 mg kg<sup>−1</sup> chitosan enhanced the protection against <italic>Aeromonas hydrophila</italic> infection. In conclusion, oral administration of dietary 7500 mg kg<sup>−1</sup> chitosan for 75 days is recommended for the survival of gibel carp.</p> </abstract>