Intertidal epilithic bacteria diversity changes along a naturally occurring carbon dioxide and pH gradient. Issue 3 (2nd July 2014)

• Record Type: Journal Article
• Authors: Taylor, Joe D.; Ellis, Rebecca; Milazzo, Marco; Hall‐Spencer, Jason M.; Cunliffe, Michael
• Is Part Of: FEMS microbiology ecology. Volume 89:Issue 3(2014)
• Page Start: 670
• Page End: 678
• Publication Date: 2014-07-02
• Subjects: Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1574-6941.12368
• Languages: English
• ISSNs: 0168-6496

Abstract

<abstract abstract-type="main" id="fem12368-abs-0001"> <title>Abstract</title> <p>Intertidal epilithic bacteria communities are important components of coastal ecosystems, yet few studies have assessed their diversity and how it may be affected by changing environmental parameters. Submarine CO<sub>2</sub> seeps produce localised areas of CO<sub>2</sub>‐enriched seawater with reduced pH levels. We utilised the seawater pH/CO<sub>2</sub> gradient at Levante Bay (Italy) to test the hypothesis that epilithic bacteria communities are modified by exposure to seawater with the varying chemical parameters. Biofilms were sampled from three sites exposed to seawater with different pH/CO<sub>2</sub> levels and diversity determined using high‐throughput sequencing of 16S rRNA genes. Seawater <italic>p</italic>CO<sub>2</sub> concentrations were increased from ambient at site 1 to 621 μatm at site 2 and 1654 μatm site 3, similar to the predicated future oceans beyond 2050 and 2150, respectively. Alpha diversity of total bacteria communities and <italic>Cyanobacteria</italic> communities was significantly different between sites (<sc>anova </sc><italic>P</italic> < 0.05). Comparison between sites showed that bacteria communities and <italic>Cyanobacteria</italic> communities were significantly different (<sc>anosim </sc><italic>P</italic> < 0.01; <sc>permanova </sc><italic>P</italic> < 0.01). <italic>Proteobacteria</italic>, <italic> Bacteroidetes</italic> and <italic>Cyanobacteria</italic> dominated all communities; however, there were differences between sites in the relative abundance of specific orders. This study provides the most detailed assessment of intertidal epilithic bacteria diversity and shows that diversity is significantly different along a seawater pH/CO<sub>2</sub> gradient. This information supports the evaluation of the impacts of future ocean acidification on coastal marine ecosystems.</p> </abstract>