The differential expression of alternatively spliced transcripts and imprinting status of MEG9 gene in cows. (24th July 2014)

• Record Type: Journal Article
• Authors: Zhang, Kun; Li, Dongjie; Wang, Mengnan; Wu, Guojiang; Shi, Yunjiao; Li, Shijie
• Is Part Of: Animal genetics. Volume 45:Number 5(2014:Oct.)
• Page Start: 660
• Page End: 664
• Publication Date: 2014-07-24
• Subjects: Animal genetics -- Periodicals; 572.8
• DOI: 10.1111/age.12195
• Languages: English
• ISSNs: 0268-9146

Abstract

<abstract abstract-type="main" id="age12195-abs-0001"> <title>Summary</title> <p> <italic>Meg9</italic>/<italic>Mirg</italic> (<italic>maternally expressed gene 9/microRNA containing gene</italic>), a non‐coding RNA (ncRNA) comprising many alternatively splicing isoforms, has been identified as maternally expressed in mouse and sheep, but its imprinting status and splicing variants are still unknown in cattle. In this study, we found three splicing variants of the cattle <italic>MEG9</italic> gene expressed in a tissue‐specific manner. A single nucleotide polymorphism site (SNP c.1354C&gt;G) was identified in exon 3 of cattle <italic>MEG9</italic> and used to distinguish between monoallelic and biallelic expression. Our results showed that <italic>MEG9</italic> exhibited monoallelic expression in all examined cattle tissues by comparing sequencing results between genomic DNA and cDNA levels at the c.1354C&gt;G SNP site, suggesting that <italic>MEG9</italic> is imprinted in cattle.</p> </abstract>