Goat casein genotypes are associated with milk production traits in the Sarda breed. (2nd July 2014)

• Record Type: Journal Article
• Authors: Vacca, G. M.; Dettori, M. L.; Piras, G.; Manca, F.; Paschino, P.; Pazzola, M.
• Is Part Of: Animal genetics. Volume 45:Number 5(2014:Oct.)
• Page Start: 723
• Page End: 731
• Publication Date: 2014-07-02
• Subjects: Animal genetics -- Periodicals; 572.8
• DOI: 10.1111/age.12188
• Languages: English
• ISSNs: 0268-9146

Abstract

<abstract abstract-type="main" id="age12188-abs-0001"> <title>Summary</title> <p>The aim of the current work was to analyze, in the Sarda breed goat, genetic polymorphism within the casein genes and to assess their influence on milk traits. Genetic variants at the <italic>CSN1S1</italic>, <italic> CSN2</italic>, <italic> CSN1S2</italic> and <italic>CSN3</italic> gene loci were investigated using PCR‐based methods, cloning and sequencing. Strong alleles prevailed at the <italic>CSN1S1</italic> gene locus and defective alleles also were revealed. Null alleles were evidenced at each calcium‐sensitive gene locus. At the <italic>CSN3</italic> gene locus, we observed a prevalence of the <italic>CSN3 A</italic> and <italic>B</italic> alleles; the occurrence of rare alleles such as <italic>CSN3 B'', C, C', D, E</italic> and <italic>M</italic>; and the <italic>CSN3 S</italic> allele (GenBank <ext-link ext-link-type="gen" xlink:href="http://www.ncbi.nlm.nih.gov/nuccore/KF644565?report=GenBank" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">KF644565</ext-link>) described here for the first time in <italic>Capra hircus</italic>. Statistical analysis showed that all genes, except <italic>CSN3</italic>, significantly influenced milk traits. The <italic>CSN1S1 BB</italic> and <italic>AB</italic> genotypes were associated with the highest percentages of protein (4.41 and 4.40 respectively) and fat (5.26 and 5.34 respectively) (<italic>P </italic><<italic> </italic>0.001). A relevant finding was that <italic>CSN2</italic> and <italic>CSN1S2</italic> genotypes affected milk protein content and yield. The polymorphism of the <italic>CSN2</italic> gene affected milk protein percentage with the highest values recorded in the <italic>CSN2 AA</italic> goats (4.35, at <italic>P </italic><<italic> </italic>0.001). The <italic>CSN1S2 AC</italic> goats provided the highest fat (51.02 g/day) and protein (41.42 g/day) (<italic>P </italic><<italic> </italic>0.01) production. This information can be incorporated into selection schemes for the Sarda breed goat.</p> </abstract>