Application and recovery of ionic liquids in the preparative separation of four flavonoids from Rhodiola rosea by on‐line three‐dimensional liquid chromatography. Issue 17 (10th July 2014)

• Record Type: Journal Article
• Authors: Ma, Shufeng; Hu, Liming; Ma, Chaoyang; Lv, Wenping; Wang, Hongxin
• Is Part Of: Journal of separation science. Volume 37:Issue 17(2014:Sep.)
• Page Start: 2314
• Page End: 2321
• Publication Date: 2014-07-10
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201400305
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A novel on‐line three‐dimensional liquid chromatography method was developed to separate four main flavonoids from <italic>Rhodiola rosea</italic>. Ethyl acetate/0.5 mol/L ionic liquid 1‐butyl‐3‐methylimidazolium chloride aqueous solution was selected as the solvent system. In the first‐dimension separation, the target flavonoids were entrapped and subsequently desorbed into the second‐dimension high‐speed countercurrent chromatographic column for separation. In the third‐dimension chromatography, the residual ionic liquid in the four separated flavonoids was removed and the used ionic liquid was recovered. As a result, 35.1 mg of compound <bold>1</bold>, 20.4 mg of compound <bold>2</bold>, 8.5 mg of compound <bold>3, </bold> and 10.6 mg of compound <bold>4</bold> were obtained from 1.53 g <italic>R. rosea</italic> extract. They were identified as rhodiosin, rhodionin, herbacetin, and kaempferol, respectively. The recovery of ionic liquid reached 99.1% of the initial amount. The results showed that this method is a powerful technology for the separation of <italic>R. rosea</italic> flavonoids and that the ionic‐liquid‐based solvent system has advantages over traditional solvent systems in renewable and environmentally friendly properties.</p> </abstract>