Quantitative Fluorescence Ratio Imaging of Intralysosomal Chloride Ions with Single Excitation/Dual Maximum Emission. Issue 37 (5th August 2014)

• Record Type: Journal Article
• Authors: Li, Ping; Zhang, Shan; Fan, Nannan; Xiao, Haibin; Zhang, Wen; Zhang, Wei; Wang, Hui; Tang, Bo
• Is Part Of: Chemistry. Volume 20:Issue 37(2014)
• Page Start: 11760
• Page End: 11767
• Publication Date: 2014-08-05
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201402999
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Fluorescence ratio imaging is currently being used to quantitatively detect biologically active molecules in biosystems; however, two excitations of most existing fluorescent ratiometric probes account for cumbersome operating conditions for imaging. Thus, a fluorescent ratiometric probe, 6‐methoxyquinolinium–dansyl (MQ‐DS), for Cl⁻ with single excitation/dual maximum emission has been developed. MQ‐DS can preferably localize into lysosomes and display excellent photostability. Upon excitation at a single wavelength, it responds precisely and instantaneously to changes in Cl⁻ concentrations, and it can be conveniently utilized to implement real‐time fluorescence ratio imaging to quantitatively track alterations in Cl⁻ levels inside cells treated under various pH conditions, and also in zebrafish with acute wounds. The successful application of the new probe in bioimaging may greatly facilitate a complete understanding of the physiological functions of Cl⁻.</p> </abstract>