High‐Throughput Bead‐Based Identification of Structure‐Switching Aptamer Beacons. Issue 13 (23rd July 2014)

• Record Type: Journal Article
• Authors: Trevino, Simon G.; Levy, Matthew
• Is Part Of: Chembiochem. Volume 15:Issue 13(2014)
• Page Start: 1877
• Page End: 1881
• Publication Date: 2014-07-23
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201402037
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We describe a new platform to identify structure‐switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence‐assisted sorting. We validated this method by isolating known and novel anti‐steroid aptamers from two separate DNA libraries that were structurally enriched with three‐way junctions. Importantly, aptamers were retrieved in only a few (three) rounds of selection by this approach and did not require further optimization, significantly streamlining the process of beacon development.</p> </abstract>