Rhinomonas nottbecki n. sp. (Cryptomonadales) and Molecular Phylogeny of the Family Pyrenomonadaceae. (7th July 2014)

• Record Type: Journal Article
• Authors: Majaneva, Markus; Remonen, Iina; Rintala, Janne‐Markus; Belevich, Ilya; Kremp, Anke; Setälä, Outi; Jokitalo, Eija; Blomster, Jaanika
• Is Part Of: Journal of eukaryotic microbiology. Volume 61:Number 5(2014:Sep./Oct.)
• Page Start: 480
• Page End: 492
• Publication Date: 2014-07-07
• Subjects: Protista -- Periodicals; Eukaryotic cells -- Periodicals; Microbiology -- Periodicals; 579.05
• DOI: 10.1111/jeu.12128
• Languages: English
• ISSNs: 1066-5234

Abstract

<abstract abstract-type="main" id="jeu12128-abs-0001"> <title>Abstract</title> <p>The cryptomonad <italic>Rhinomonas nottbecki</italic> n. sp., isolated from the Baltic Sea, is described from live and fixed cells studied by light, scanning, and transmission electron microscopy together with sequences of the partial nucleus‐ and nucleomorph‐encoded 18S rRNA genes as well as the nucleus‐encoded ITS1, 5.8S, ITS2, and the 5′‐end of the 28S rRNA gene regions. The sequence analyses include comparison with 43 strains from the family Pyrenomonadaceae. <italic>Rhinomonas nottbecki</italic> cells are dorsoventrally flattened, obloid in shape; 10.0–17.2 μm long, 5.5–8.1 μm thick, and 4.4–8.8 μm wide. The inner periplast has roughly hexagonal plates. <italic>Rhinomonas nottbecki</italic> cells resemble those of <italic>Rhinomonas reticulata</italic>, but the nucleomorph 18S rRNA gene of <italic>R. nottbecki</italic> differs by 2% from that of <italic>R. reticulata</italic>, while the ITS region by 11%. The intraspecific variability in the ITS region of <italic>R. nottbecki</italic> is 5%. In addition, the predicted ITS2 secondary structures are different in <italic>R. nottbecki</italic> and <italic>R. reticulata</italic>. The family Pyrenomonadaceae includes three clades: Clade A, Clade B, and Clade C. All <italic>Rhinomonas</italic> sequences branched within the Clade C, while the genus <italic>Rhodomonas</italic> is paraphyletic. The analyses suggest that the genus <italic>Storeatula</italic> is an alternating morphotype of the genera <italic>Rhinomonas</italic> and <italic>Rhodomonas</italic> and that the family Pyrenomonadaceae includes some species that were described multiple times, as well as novel species.</p> </abstract>