Exploring Anopheles gut bacteria for Plasmodium blocking activity. (24th February 2014)

• Record Type: Journal Article
• Authors: Bahia, Ana C.; Dong, Yuemei; Blumberg, Benjamin J.; Mlambo, Godfree; Tripathi, Abhai; BenMarzouk‐Hidalgo, Omar J.; Chandra, Ramesh; Dimopoulos, George
• Is Part Of: Environmental microbiology. Volume 16:Number 9(2014:Sep.)
• Page Start: 2980
• Page End: 2994
• Publication Date: 2014-02-24
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.12381
• Languages: English
• ISSNs: 1462-2912

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Malaria parasite transmission requires the successful development of <italic>P</italic><italic>lasmodium</italic> gametocytes into flagellated microgametes upon mosquito blood ingestion, and the subsequent fertilization of microgametes and macrogametes for the development of motile zygotes, called ookinetes, which invade and transverse the <italic>A</italic><italic>nopheles</italic> vector mosquito midgut at around 18–36 h after blood ingestion. Within the mosquito midgut, the malaria parasite has to withstand the mosquito's innate immune response and the detrimental effect of its commensal bacterial flora. We have assessed the midgut colonization capacity of five gut bacterial isolates from field‐derived, and two from laboratory colony, mosquitoes and their effect on <italic>P</italic><italic>lasmodium </italic>development <italic>in vivo</italic> and <italic>in vitro</italic>, along with their impact on mosquito survival. Some bacterial isolates activated the mosquito's immune system, affected the mosquito's lifespan, and were capable of blocking <italic>P</italic><italic>lasmodium </italic>development. We have also shown that the ability of these bacteria to inhibit the parasites is likely to involve different mechanisms and factors. A <italic>S</italic><italic>erratia marcescens</italic> isolate was particularly efficient in colonizing the mosquitoes' gut, compromising mosquito survival and inhibiting both <italic>Plasmodium</italic> sexual‐ and asexual‐stage through secreted factors, thereby rendering it a potential candidate for the development of a malaria transmission intervention strategy.</p> </abstract>