A combining signal amplification of atom transfer radical polymerization and redox polymerization for visual biomolecules detection. Issue 19 (22nd July 2014)

• Record Type: Journal Article
• Authors: Zhuang, Dequan; Shen, Heyun; Liu, Guodong; Yu, Changyuan; Yang, Jing
• Is Part Of: Journal of polymer science. Volume 52:Issue 19(2014:Oct.)
• Page Start: 2791
• Page End: 2799
• Publication Date: 2014-07-22
• Subjects: 547
• DOI: 10.1002/pola.27303
• Languages: English
• ISSNs: 0887-624X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>A novel combination of atom transfer radical polymerization (ATRP) and redox polymerization is here used to allow instrument‐free visualization of special biomolecules for which dynamic polymer growth is used in signal amplification. In this method, the convenient and mild redox polymerization‐assisted amplification with cerium ammonium (IV) nitrate as oxidant at the second stage was achieved by directly using the hydroxyl groups from poly(hydroxyethyl methacrylate) (PHEMA) synthesized via ATRP at the first stage. The brushed polymers poly(hydroxylethyl methacrylate)‐<italic>branched</italic>‐poly (acrylamide) (PHEMA‐<italic>branched</italic>‐PAM) prepared by successive ATRP and redox polymerization <italic>in situ</italic> drastically grew up at the detected biomolecules spot to improve the visibility of biomolecule and simplify the detection procedure. With the proposed strategy, the signal amplification of streptavidin (SA) as model detected biomolecule was investigated on two different substrates such as silicon wafer and gold, respectively. As a result, detection limit of SA was demonstrated on the gold substrates where binding of 1.0 ng/mL SA was differentiable from the background using ellipsometry. Moreover, binding of 0.5 nmol/L DNA led to visually distinguishable spots on the gold surface under mild condition. The proposed method exhibited an efficient amplification performance for molecules detection, and paved a new way for visual diagnosis of biomolecules. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. <bold>2014</bold>, <italic>52</italic>, 2791–2799</p> </abstract>