Conserved nucleation sites reinforce the significance of Phi value analysis in protein‐folding studies. Issue 7 (18th July 2014)

• Record Type: Journal Article
• Authors: Gianni, Stefano; Jemth, Per
• Is Part Of: IUBMB life. Volume 66:Issue 7(2014:Jul.)
• Page Start: 449
• Page End: 452
• Publication Date: 2014-07-18
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; 572.8
• DOI: 10.1002/iub.1287
• Languages: English
• ISSNs: 1521-6543

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The only experimental strategy to address the structure of folding transition states, the so‐called Φ value analysis, relies on the synergy between site directed mutagenesis and the measurement of reaction kinetics. Despite its importance, the Φ value analysis has been often criticized and its power to pinpoint structural information has been questioned. In this hypothesis, we demonstrate that comparing the Φ values between proteins not only allows highlighting the robustness of folding pathways but also provides <italic>per se</italic> a strong validation of the method. © 2014 IUBMB Life, 66(7):449–452, 2014</p> </abstract>