Analysis of centromere signal patterns in breast cancer cells with chromosomal instability using image cytometry combined with centromere fluorescence in situ hybridization. Issue 9 (9th July 2014)

• Record Type: Journal Article
• Authors: Ito, Hideaki; Oga, Atsunori; Ikemoto, Kenzo; Furuya, Tomoko; Maeda, Noriko; Yamamoto, Shigeru; Kawauchi, Shigeto; Itoh, Hiroshi; Oka, Masaaki; Sasaki, Kohsuke
• Is Part Of: Cytometry. Volume 85:Issue 9(2014:Sep.)
• Page Start: 809
• Page End: 816
• Publication Date: 2014-07-09
• Subjects: Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.22502
• Languages: English
• ISSNs: 1552-4922

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Fluorescence <italic>in situ</italic> hybridization (FISH) with centromeric probes is a method used to detect chromosomal instability (CIN), a hallmark of most cancers. However, no studies thus far have investigated the relationship between centromeric FISH signals and the cell cycle in cancer cells. In this study, the chromosome content in each cell cycle phase was evaluated with respect to the number of centromeric FISH signals in two breast cancer cell lines and eight surgically resected breast cancer specimens using image cytometry. Variations in chromosome number were detected at each phase of the cell cycle but were not associated with proliferative capacity in the cell lines. Furthermore, the chromosome doubling frequency differed in each cell line and clinical specimen. These results reveal two aspects of centromeric FISH signal variation in breast cancers that exhibit CIN, and suggest that chromosome doubling is a remarkable occurrence that may increase the heterogeneity of tumors. © 2014 International Society for Advancement of Cytometry</p> </abstract>