Effect of vitamin D3 on behavioural and biochemical parameters in diabetes type 1‐induced rats. (19th June 2014)

• Record Type: Journal Article
• Authors: Calgaroto, Nicéia Spanholi; Thomé, Gustavo Roberto; da Costa, Pauline; Baldissareli, Jucimara; Hussein, Fátima Abdala; Schmatz, Roberta; Rubin, Maribel A.; Signor, Cristiane; Ribeiro, Daniela Aymone; Carvalho, Fabiano Barbosa; de Oliveira, Lizielle Souza; Pereira, Luciane Belmonte; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina
• Is Part Of: Cell biochemistry and function. Volume 32:Number 6(2013:Aug.)
• Page Start: 502
• Page End: 510
• Publication Date: 2014-06-19
• Subjects: Cytochemistry -- Periodicals; Cell metabolism -- Periodicals; Biochemistry -- Periodicals; Cytology -- Periodicals; 572
• DOI: 10.1002/cbf.3044
• Languages: English
• ISSNs: 0263-6484

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Diabetes is associated with long‐term complications in the brain and reduced cognitive ability. Vitamin D<sub>3</sub> (VD<sub>3</sub>) appears to be involved in the amelioration of hyperglycaemia in streptozotocin (STZ)‐induced diabetic rats. Our aim was to analyse the potential of VD<sub>3</sub> in avoiding brain damage through evaluation of acetylcholinesterase (AChE), Na<sup>+</sup>K<sup>+</sup>‐adenosine triphosphatase (ATPase) and delta aminolevulinate dehydratase (<italic>δ</italic>‐ALA‐D) activities and thiobarbituric acid reactive substance (TBARS) levels from cerebral cortex, as well as memory in STZ‐induced diabetic rats. Animals were divided into eight groups (<italic>n</italic> = 5): control/saline, control/metformin (Metf), control/VD<sub>3</sub>, control/Metf + VD<sub>3</sub>, diabetic/saline, diabetic/Metf, diabetic/VD<sub>3</sub> and diabetic/Metf + VD<sub>3</sub>. Thirty days after treatment, animals were submitted to contextual fear‐conditioning and open‐field behavioural tests, after which they were sacrificed and the cerebral cortex was dissected. Our results demonstrate a significant memory deficit, an increase in AChE activity and TBARS levels and a decrease in <italic>δ</italic>‐ALA‐D and Na<sup>+</sup>K<sup>+</sup>‐ATPase activities in diabetic rats when compared with the controls. Treatment of diabetic rats with Metf and VD<sub>3</sub> prevented the increase in AChE activity when compared with the diabetic/saline group. In treated diabetic rats, the decrease in Na<sup>+</sup>K<sup>+</sup>‐ATPase was reverted when compared with non‐treated rats, but the increase in <italic>δ</italic>‐ALA‐D activity was not. VD<sub>3</sub> prevented diabetes‐induced TBARS level and improved memory. Our results show that VD<sub>3</sub> can avoid cognitive deficit through prevention of changes in important enzymes such as Na<sup>+</sup>K<sup>+</sup>‐ATPase and AChE in cerebral cortex in type 1 diabetic rats. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>