POTATO LEAF EXTRACT AND ITS COMPONENT, α‐SOLANINE, EXERT SIMILAR IMPACTS ON DEVELOPMENT AND OXIDATIVE STRESS IN Galleria mellonella L. Issue 1 (8th July 2014)

• Record Type: Journal Article
• Authors: Adamski, Zbigniew; Adamski, Zbigniew; Marciniak, Pawel; Ziemnicki, Kazimierz; Büyükgüzel, Ender; Erdem, Meltem; Büyükgüzel, Kemal; Ventrella, Emanuela; Falabella, Patrizia; Cristallo, Massimo; Salvia, Rosanna; Bufo, Sabino Aurelio; Scrano, Laura
• Is Part Of: Archives of insect biochemistry and physiology. Volume 87:Issue 1(2014:Sep.)
• Page Start: 26
• Page End: 39
• Publication Date: 2014-07-08
• Subjects: Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21177
• Languages: English
• ISSNs: 0739-4462

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Plants synthesize a broad range of secondary metabolites that act as natural defenses against plant pathogens and herbivores. Among these, potato plants produce glycoalkaloids (GAs). In this study, we analyzed the effects of the dried extract of fresh potato leaves (EPL) on the biological parameters of the lepidopteran, <italic>Galleria mellonella</italic> (L.) and compared its activity to one of the main EPL components, the GA α‐solanine. Wax moth larvae were reared from first instar on a diet supplemented with three concentrations of EPL or α‐solanine. Both EPL and α‐solanine affected survivorship, fecundity, and fertility of <italic>G. mellonella</italic> to approximately the same extent. We evaluated the effect of EPL and α‐solanine on oxidative stress in midgut and fat body by measuring malondialdehyde (MDA) and protein carbonyl (PCO) contents, both biomarkers of oxidative damage. We evaluated glutathione S‐transferase (GST) activity, a detoxifying enzyme acting in prevention of oxidative damage. EPL and α‐solanine altered MDA and PCO concentrations and GST activity in fat body and midgut. We infer that the influence of EPL on <italic>G. mellonella</italic> is not enhanced by synergistic effects of the totality of potato leaf components compared to α‐solanine alone.</p> </abstract>