Metabolic engineering of Bacillus amyloliquefaciens for poly‐gamma‐glutamic acid (γ‐PGA) overproduction. Issue 5 (1st July 2014)

• Record Type: Journal Article
• Authors: Feng, Jun; Gu, Yanyan; Sun, Yang; Han, Lifang; Yang, Chao; Zhang, Wei; Cao, Mingfeng; Song, Cunjiang; Gao, Weixia; Wang, Shufang
• Is Part Of: Microbial biotechnology. Volume 7:Issue 5(2014:Sep.)
• Page Start: 446
• Page End: 455
• Publication Date: 2014-07-01
• Subjects: Microbial biotechnology -- Periodicals; Biotechnology; Microbiology; 660.62
• DOI: 10.1111/1751-7915.12136
• Languages: English
• ISSNs: 1751-7915

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>We constructed a metabolically engineered glutamate‐independent <italic>B</italic><italic>acillus amyloliquefaciens</italic> strain with considerable γ‐PGA production. It was carried out by double‐deletion of the <italic>cwl</italic><italic>O</italic> gene and <italic>eps</italic><italic>A</italic><italic>‐</italic><italic>O</italic> cluster, as well as insertion of the <italic>vgb</italic> gene in the bacteria chromosome. The final generated strain NK‐PV elicited the highest production of γ‐PGA (5.12 g l⁻¹), which was 63.2% higher than that of the wild‐type NK‐1 strain (3.14 g l⁻¹). The γ‐PGA purity also improved in the NK‐PV strain of 80.4% compared with 76.8% for the control. Experiments on bacterial biofilm formation experiment showed that NK‐1 and NK‐c (Δ<italic>cwl</italic><italic>O</italic>) strains can form biofilm; the <italic>eps</italic><italic>A</italic><italic>‐</italic><italic>O</italic> deletion NK‐7 and NK‐PV strains could only form an incomplete biofilm.</p> </abstract>