Multiparametric optical and MR imaging demonstrate inhibition of tumor angiogenesis natural history by mural cell therapy. Issue 3 (7th October 2013)

• Record Type: Journal Article
• Authors: Faye, Nathalie; Clément, Olivier; Balvay, Daniel; Fitoussi, Victor; Pidial, Laetitia; Sandoval, Federico; Autret, Gwennhael; Silvestre, Jean Sébastien; Dean, Carole Leré; Alison, Marianne; Fournier, Laure S.
• Is Part Of: Magnetic resonance in medicine. Volume 72:Issue 3(2014:Sep.)
• Page Start: 841
• Page End: 849
• Publication Date: 2013-10-07
• Subjects: Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.24970
• Languages: English
• ISSNs: 0740-3194

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mrm24970-sec-0001" sec-type="section"> <title>Purpose</title> <p>To determine whether functional imaging using MRI and fibered confocal fluorescence microscopy (FCFM) could be used to monitor cell therapy by mural progenitor cells (MPC).</p> </sec> <sec id="mrm24970-sec-0002" sec-type="section"> <title>Methods</title> <p>Fifty mice bearing TC1 murine xenograft tumors were allocated into: control (n = 17), sham (phosphate buffer saline, n = 16), and MPC‐treated (MPC, n = 17) groups. MRI was performed before (D<sub>0</sub>) and 7 days (D<sub>7</sub>) after injection measuring tumor size, R<sub>2</sub>* under air, oxygen, and carbogen using blood oxygen level dependent (BOLD) and f (fraction linked to microcirculation), D* (perfusion related coefficient) and Dr (restricted diffusion coefficient) using diffusion‐weighted sequences based on the IVIM (intravoxel incoherent motion) method. FCFM was performed at D<sub>7</sub> measuring "index leakage" (capillary permeability).</p> </sec> <sec id="mrm24970-sec-0003" sec-type="section"> <title>Results</title> <p>Tumor growth was significantly slowed down in the MPC‐treated animals (<italic>P</italic> = 0.002) on D<sub>7</sub>. R<sub>2</sub>*<sub>air</sub> significantly decreased in controls between D<sub>0</sub> and D<sub>7</sub> (<italic>P</italic> = 0.03), reflecting a decrease in tumor oxygenation. ΔR<sub>2</sub>*<sub>O2CO2</sub> significantly increased in controls between D<sub>0</sub> and D<sub>7</sub> (<italic>P</italic> = 0.01) reflecting loss of vessel response to carbogen. D* significantly decreased in controls between D<sub>0</sub> and D<sub>7</sub> (<italic>P</italic> = 0.03). Finally, "index leakage" was lower in the MPC‐treated tumors (<italic>P</italic> = 0, 009).</p> </sec> <sec id="mrm24970-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Treatment by MPC resulted in slowing down of tumor growth, capillary permeability decrease, and stabilization of tumor angiogenesis. Magn Reson Med 72:841–849, 2014. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>