Comparison of UVB and UVC Effects on the DNA Damage‐Response Protein 53BP1 in Human Pancreatic Cancer. Issue 10 (October 2014)

• Record Type: Journal Article
• Authors: Uehara, Fuminari; Miwa, Shinji; Tome, Yasunori; Hiroshima, Yukihiko; Yano, Shuya; Yamamoto, Mako; Efimova, Elena; Matsumoto, Yasunori; Maehara, Hiroki; Bouvet, Michael; Kanaya, Fuminori; Hoffman, Robert M.
• Is Part Of: Journal of cellular biochemistry. Volume 115:Issue 10(2014:Oct.)
• Page Start: 1724
• Page End: 1728
• Publication Date: 2014-10
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.24837
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb24837-sec-0001" sec-type="section"> <p>We have previously demonstrated that ultraviolet (UV) light is effective against a variety of cancer cells expressing fluorescent proteins in vivo as well as in vitro. In the present report, we compared the DNA damage repair (DDR) response of pancreatic cancer cells after UVB or UVC irradiation. The UV‐induced DNA damage repair was imaged with green fluorescent protein (GFP) fused to the DDR‐related chromatin‐binding protein 53BP1 in MiaPaCa‐2 human pancreatic cancer cells growing in 3D Gelfoam® histoculture and in superficial tumors grown in nude mice. 53BP1‐GFP forms foci during DNA damage repair. A clonogenic assay in 2D monolayer culture initially showed that UVC and UVB inhibited MiaPaCa‐2 cell proliferation in a dose‐dependent manner, with UVC having more efficacy. Three‐dimensional Gelfoam® histocultures and confocal imaging enabled 53BP1‐GFP foci to be observed within 1 h after UV irradiation, indicating the onset of DDR response. UVB‐induced 53BP1‐GFP focus formation was observed up to a depth of 120 µm in MiaPaCa‐2 cells on Gelfoam® compared to 80 µm for UVC. UVB‐induced 53BP1‐GFP focus formation was observed up to a depth of 80 µm in MiaPaCa‐2 cells, implanted within skin flaps in mice, at a significantly greater extent than UVC. MiaPaCa‐2 cells irradiated by UVB or UVC in the skin‐flap mouse model had a significant decrease in tumor growth compared to untreated controls with UVB having more efficacy than UVC. Our results demonstrate that UVB has greater tissue penetration than UVC because of its longer wavelength and has clinical potential for eradicating superficial cancer. J. Cell. Biochem. 115: 1724–1728, 2014. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>