Development of New PCR Primers by Comparative Genomics for the Detection of Helicobacter suis in Gastric Biopsy Specimens. Issue 4 (28th March 2014)

• Record Type: Journal Article
• Authors: Matsui, Hidenori; Takahashi, Tetsufumi; Murayama, Somay Y.; Uchiyama, Ikuo; Yamaguchi, Katsushi; Shigenobu, Shuji; Matsumoto, Takehisa; Kawakubo, Masatomo; Horiuchi, Kazuki; Ota, Hiroyoshi; Osaki, Takako; Kamiya, Shigeru; Smet, Annemieke; Flahou, Bram; Ducatelle, Richard; Haesebrouck, Freddy; Takahashi, Shinichi; Nakamura, Shinichi; Nakamura, Masahiko
• Is Part Of: Helicobacter. Volume 19:Issue 4(2014:Aug.)
• Page Start: 260
• Page End: 271
• Publication Date: 2014-03-28
• Subjects: Helicobacter -- Periodicals; Helicobacter infections -- Periodicals; Stomach -- Diseases -- Periodicals; 616.3301405
• DOI: 10.1111/hel.12127
• Languages: English
• ISSNs: 1083-4389

Abstract

<abstract abstract-type="main" id="hel12127-abs-0001"> <title>Abstract</title> <sec id="hel12127-sec-0001" sec-type="section"> <title>Background</title> <p>Although the infection rate of <italic>Helicobacter suis</italic> is significantly lower than that of <italic>Helicobacter pylori</italic>, the <italic>H. suis</italic> infection is associated with a high rate of gastric mucosa‐associated lymphoid tissue (MALT) lymphoma. In addition, in vitro cultivation of <italic>H. suis</italic> remains difficult, and some <italic>H. suis</italic>‐infected patients show negative results on the urea breath test (UBT).</p> </sec> <sec id="hel12127-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Female C57BL/6J mice were orally inoculated with mouse gastric mucosal homogenates containing <italic>H. suis</italic> strains TKY or SNTW101 isolated from a cynomolgus monkey or a patient suffering from nodular gastritis, respectively. The high‐purity chromosomal DNA samples of <italic>H. suis</italic> strains TKY and SNTW101 were prepared from the infected mouse gastric mucosa. The SOLiD sequencing of two <italic>H. suis</italic> genomes enabled comparative genomics of 20 <italic>Helicobacter</italic> and 11 <italic>Campylobacter</italic> strains for the identification of the <italic>H. suis</italic>‐specific nucleotide sequences.</p> </sec> <sec id="hel12127-sec-0003" sec-type="section"> <title>Results</title> <p>Oral inoculation with mouse gastric mucosal homogenates containing <italic>H. suis</italic> strains TKY and SNTW101 induced gastric MALT lymphoma and the formation of gastric lymphoid follicles, respectively, in C57BL/6J mice. Two conserved nucleotide sequences among six <italic>H. suis</italic> strains were identified and were used to design diagnostic PCR primers for the detection of <italic>H. suis</italic>.</p> </sec> <sec id="hel12127-sec-0004" sec-type="section"> <title>Conclusions</title> <p>There was a strong association between the <italic>H. suis</italic> infection and gastric diseases in the C57BL/6 mouse model. PCR diagnosis using an <italic>H. suis</italic>‐specific primer pair is a valuable method for detecting <italic>H. suis</italic> in gastric biopsy specimens.</p> </sec> </abstract>