Effective antidiabetic and antioxidant fractions of Otostegia persica extract and their constituents. (August 2014)

• Record Type: Journal Article
• Authors: Tofighi, Zahra; Alipour, Fatemeh; Hadavinia, Hormoz; Abdollahi, Mohammad; Hadjiakhoondi, Abbas; Yassa, Narguess
• Is Part Of: Pharmaceutical biology. Volume 52:Number 8(2014:Aug.)
• Page Start: 961
• Page End: 966
• Publication Date: 2014-08
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.874463
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: <italic>Otostegia persica</italic> (Burm.) Boiss. (Lamiaceae), "Goldar" in Persian, is widely used in the folk medicine of south Iran for control of diabetes mellitus.</p> <p> <italic>Objective</italic>: In the present study, hypoglycemic and antioxidant effects of different fractions of the <italic>O. persica</italic> extract were investigated and constituents of effective fractions were elucidated.</p> <p> <italic>Materials and method</italic>: Different concentrations (100–400 mg/kg) of aqueous infusion (AI) of flowering aerial parts of the plant (traditional preparation) and all fractions of the <italic>O. persica</italic> extract (i.p. injection) were tested for antidiabetic activity in streptozocin-induced diabetic NMRI mice. Blood glucose level was measured at time 0 and intervals of 1, 2, 4, and 6 h later. Antioxidant activities of different fractions of the plant extract and pure compounds (0.1, 0.5, and 1 mg/ml) were determined with the DPPH method. Four compounds were isolated and identified from potent fractions.</p> <p> <italic>Results and discussion</italic>: Antidiabetic activity demonstrated that the effect of the methanol fraction at a dose of 300 mg/kg was equivalent with glibenclamide, and at a dose of 400 mg/kg was comparable with glibenclamide and insulin (<italic>p</italic> > 0.05). The EC<sub>50</sub> of the methanol fraction was 307.12 mg. Methanol and ethyl acetate fractions showed antioxidant activities (both IC<sub>50</sub> equal to 0.49 mg/ml), so these fractions were selected for the purification of compounds. Chrysoeriol from ethyl acetate and three apigenin derivatives (6-methylapigenin, apigenin-7-<italic>O</italic>-glucoside, and echinaticin) from the methanol fraction were isolated and identified (new for the species). Chrysoeriol exhibited potent antioxidant activity comparable with vitamin E and BHT (<italic>p</italic> > 0.05).</p> <p> <italic>Conclusion</italic>: The present study confirmed the folklore usage of <italic>O. persica</italic> for antidiabetic properties.</p> </abstract>