Screening compounds of Chinese medicinal herbs anti-Marek's disease virus. (July 2014)

• Record Type: Journal Article
• Authors: Sun, Yaogui; Niu, Li; Song, Meiqin; Zhao, Xin; Sun, Na; He, Junping; Wu, Caihong; Jiang, Junbing; Bai, Yuansheng; Guo, Jianhua; Li, Hongquan
• Is Part Of: Pharmaceutical biology. Volume 52:Number 7(2014:Jul.)
• Page Start: 841
• Page End: 847
• Publication Date: 2014-07
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.871639
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Marek's disease (MD) seriously threatens the world poultry industry and has resulted in great economic losses. Chinese medicinal herbs are a rich source for lead compounds and drug candidates for antiviral treatments.</p> <p> <italic>Objective</italic>: To investigate the anti-MDV activity and mechanism of 20 compounds extracted from Chinese medicinal herbs.</p> <p> <italic>Materials and methods</italic>: Antiviral assay, time of addition experiments, and virucidal assay were performed on chicken embryo fibroblast cells. The 50% cytotoxic concentration and 50% effective concentration were determined and, accordingly, selectivity index and inhibition ratio were calculated.</p> <p> <italic>Results</italic>: Antiviral assay showed dipotassium glycyrrhizinate (DG) and sodium tanshinone IIA sulfonate (STS) exhibited significantly inhibitory activity against MDV in a dose-dependent manner. EC<sub>50</sub> of DG and STS were 893.5 ± 36.99 µg/mL and 54.82 ± 2.99 µg/mL, and selective index (SI) were >3.36 and >9.12, respectively. Time of addition experiment and virucidal assay demonstrated DG inhibited viral replication in the full replication cycle and inactivated MDV particles in non-time-dependent manner, but STS interfered with the early stage of MDV replication and inactivated MDV particles in a time-dependent manner. Moreover, both DG and STS promoted apoptosis of cells infected by MDV.</p> <p> <italic>Discussion and conclusion</italic>: DG and STS have great potential for developing new anti-MDV drugs for clinic application.</p> </abstract>