Variant alleles of VEGF and risk of esophageal cancer and lymph node metastasis. (May 2014)

• Record Type: Journal Article
• Authors: Gu, Haiyong; Qiu, Wanshan; Shi, Yijun; Chen, Suocheng; Yin, Jun
• Is Part Of: Biomarkers. Volume 19:Number 3(2014)
• Page Start: 252
• Page End: 258
• Publication Date: 2014-05
• Subjects: Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2014.902997
• Languages: English
• ISSNs: 1354-750X

Abstract

<abstract> <title>Abstract</title> <p>Condensed abstract: The variant alleles of the functional polymorphisms, <italic>VEGF</italic> −2578 C > A, −1498 T > C and +936 C > T, were not associated with risk of esophageal cancer and lymph node metastasis. Compared with the most common haplotype, C<sub>−2578</sub>T<sub>−1498</sub>C<sub>+936</sub>, the A<sub>−2578</sub>C<sub>−1498</sub>C<sub>+936</sub> haplotype was associated with a borderline significantly increased risk of esophageal cancer. C<sub>−2578</sub>C<sub>−1498</sub>C<sub>+936</sub> and A<sub>−2578</sub>T<sub>−1498</sub>T<sub>+936</sub> haplotypes were associated with significantly increased risk of esophageal cancer.</p> <p>Vascular endothelial growth factor (VEGF) is a potent stimulator for angiogenesis. It has been implicated in the growth and metastasis of esophageal cancer. Three functional single nucleotide polymorphisms (SNPs) of <italic>VEGF</italic> (−2578 C > A, −1498 T > C and +936 C > T) are known to be related to VEGF expression. We conducted a case–control study to evaluate the effects of these three functional SNPs on the development of esophageal cancer and lymph node metastasis. A total of 497 cases and 380 controls were analyzed. Genotypes were determined by matrix assisted laser desorption/ionization time-of-flight mass spectrometry and direct sequence methods. The variant alleles of the functional polymorphisms <italic>VEGF</italic> −2578 C > A, −1498 T > C and +936 C > T SNPs were not associated with esophageal cancer risk. These <italic>VEGF</italic> genotypes were not associated with the risk of esophageal cancer after stratification. Furthermore, no association was observed between <italic>VEGF</italic> −2578 C > A, −1498 T > C and +936 C > T polymorphisms and lymph node metastasis. Compared with the most common haplotype C<sub>−2578</sub>T<sub>−1498</sub>C<sub>+936</sub>, the A<sub>−2578</sub>C<sub>−1498</sub>C<sub>+936</sub> haplotype was associated with a borderline significantly increased risk of esophageal cancer. C<sub>−2578</sub>C<sub>−1498</sub>C<sub>+936</sub> and A<sub>−2578</sub>T<sub>−1498</sub>T<sub>+936</sub> haplotypes were associated with significantly increased risk of esophageal cancer. Variants in the functional polymorphisms of <italic>VEGF</italic> may not contribute to esophageal cancer and lymph node metastasis susceptibility. <italic>VEGF</italic> A<sub>−2578</sub>C<sub>−1498</sub>C<sub>+936, </sub> C<sub>−2578</sub>C<sub>−1498</sub>C<sub>+936</sub> and A<sub>−2578</sub>T<sub>−1498</sub>T<sub>+936</sub> haplotypes may be associated with increased risk of esophageal cancer. However, our results were obtained with a limited sample size and therefore this is a preliminary conclusion. Validation by a larger study with more diverse ethnic populations is needed.</p> </abstract>