Porcine brain microvessel endothelial cells show pro-inflammatory response to the size and composition of metallic nanoparticles. (May 2014)

• Record Type: Journal Article
• Authors: Trickler, William J.; Lantz-McPeak, Susan M.; Robinson, Bonnie L.; Paule, Merle G.; Slikker, William; Biris, Alexandru S.; Schlager, John J.; Hussain, Saber M.; Kanungo, Jyotshna; Gonzalez, Carmen; Ali, Syed F.
• Is Part Of: Drug metabolism reviews. Volume 46:Number 2(2014:Apr.)
• Page Start: 224
• Page End: 231
• Publication Date: 2014-05
• Subjects: Drugs -- Metabolism -- Periodicals; Pharmacokinetics -- Periodicals; Pharmaceutical Preparations -- metabolism -- Periodicals; 615
• DOI: 10.3109/03602532.2013.873450
• Languages: English
• ISSNs: 0360-2532

Abstract

<abstract> <title>Abstract</title> <p>The purpose of the current studies was to determine if systemic exposure of various metallic nanoparticles differing in size and composition [silver (Ag-NPs, 25, 40 and 80 nm), copper-oxide (Cu-NPs, 40 and 60 nm) or gold (Au-NPs, 3 and 5 nm)] can induce the release of pro-inflammatory mediators that influence the restrictive nature of the blood–brain barrier (BBB) <italic>in vitro.</italic> Confluent porcine brain microvessel endothelial cells (pBMECs) (8–12 days) were treated with various metallic nanoparticles (15 μg/ml). Extracellular concentrations of pro-inflammatory mediators (IL-1β, TNFα and PGE<sub>2</sub>) were evaluated using ELISA. pBMECs were cultured in standard 12-well Transwell® inserts, and permeability was evaluated by measuring the transport of fluorescein across the pBMEC monolayers. PGE<sub>2</sub> release following Cu-NP exposure was significantly increased when compared to the control. Similar results were observed for Ag-NPs but not Au-NPs. The secretion of TNFα and IL-1β was observed for both Cu-NPs and Ag-NPs but not in response to Au-NPs. The post-treatment time profiles of TNFα and IL-1β revealed that the IL-1β response was more persistent. The permeability ratios (exposure/control) were significantly greater following exposure to Cu-NPs or Ag-NPs, compared to Au-NPs. Together, these data suggest that the composition and size of NPs can cause significant pro-inflammatory response that can influence the integrity of the BBB.</p> </abstract>