Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO‐dependent Stn1 telomere association. (12th June 2014)

• Record Type: Journal Article
• Authors: Garg, Mansi; Gurung, Resham L; Mansoubi, Sahar; Ahmed, Jubed O; Davé, Anoushka; Watts, Felicity Z; Bianchi, Alessandro
• Is Part Of: EMBO reports. Volume 15:Number 8(2014:Aug.)
• Page Start: 871
• Page End: 877
• Publication Date: 2014-06-12
• Subjects: Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.201438919
• Languages: English
• ISSNs: 1469-221X

Abstract

<abstract abstract-type="main" id="embr201438919-abs-0001"> <title>Abstract</title> <p>Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang‐binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang‐binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association, and a SUMO‐Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.</p> </abstract>