Association of serum interleukin‐27 with the exacerbation of chronic obstructive pulmonary disease. Issue 7 (3rd July 2014)

• Record Type: Journal Article
• Authors: Angata, Takashi; Ishii, Takeo; Gao, Congxiao; Ohtsubo, Kazuaki; Kitazume, Shinobu; Gemma, Akihiko; Kida, Kozui; Taniguchi, Naoyuki
• Is Part Of: Physiological reports. Volume 2:Issue 7(2014:Jul.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2014-07-03
• Subjects: Physiology -- Periodicals; 571
• DOI: 10.14814/phy2.12069
• Languages: English
• ISSNs: 2051-817X

Abstract

<abstract abstract-type="main" id="phy212069-abs-0001"> <title>Abstract</title> <p>We have previously demonstrated that chronic obstructive pulmonary disease (COPD) patients who do not have Siglec‐14 are less prone to exacerbation of the disease. Siglec‐14 is a myeloid cell protein that recognizes bacteria and triggers inflammatory responses. Therefore, soluble mediators secreted by myeloid cells responding to Siglec‐14 engagement could be involved in the pathogenesis of exacerbation and could potentially be utilized as biomarkers of exacerbation. To find out, we sought genes specifically induced in Siglec‐14<sup>+</sup> myeloid cells and evaluated their utility as biomarkers of COPD exacerbation. Using DNA microarray, we compared gene expression levels in Siglec‐14<sup>+</sup> and control myeloid cell lines stimulated with or without nontypeable <italic>Haemophilus influenzae</italic> to select genes that were specifically induced in Siglec‐14<sup>+</sup> cells. The expressions of several cytokine and chemokine genes were specifically induced in Siglec‐14<sup>+</sup> cells. The concentrations of seven gene products were analyzed by multiplex bead array assays in paired COPD patient sera (<italic>n</italic> = 39) collected during exacerbation and stable disease states. Those gene products that increased during exacerbation were further tested using an independent set (<italic>n</italic> = 32) of paired patient sera. Serum concentration of interleukin‐27 (IL‐27) was elevated during exacerbation (discovery set: <italic>P</italic> = 0.0472; verification set: <italic>P</italic> = 0.0428; combined: <italic>P</italic> = 0.0104; one‐sided Wilcoxon matched‐pairs signed‐rank test), particularly in exacerbations accompanied with sputum purulence and in exacerbations lasting more than a week. We concluded that IL‐27 might be mechanistically involved in the exacerbation of COPD and could potentially serve as a systemic biomarker of exacerbation.</p> </abstract>