Bacteria isolated from roots and rhizosphere of Vitis vinifera retard water losses, induce abscisic acid accumulation and synthesis of defense‐related terpenes in in vitro cultured grapevine. Issue 4 (4th November 2013)

• Record Type: Journal Article
• Authors: Salomon, María Victoria; Bottini, Rubén; de Souza Filho, Gonçalo Apolinário; Cohen, Ana Carmen; Moreno, Daniela; Gil, Mariana; Piccoli, Patricia
• Is Part Of: Physiologia plantarum. Volume 151:Issue 4(2014:Aug.)
• Page Start: 359
• Page End: 374
• Publication Date: 2013-11-04
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/ppl.12117
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main" id="ppl12117-abs-0001"> <title>Abstract</title> <p id="ppl12117-para-0001">Eleven bacterial strains were isolated at different soil depths from roots and rhizosphere of grapevines from a commercial vineyard. By 16S rRNA gene sequencing 10 different genera and 8 possible at species level were identified. From them, <italic>Bacillus licheniformis</italic> Rt4M10 and <italic>Pseudomonas fluorescens</italic> Rt6M10 were selected according to their characteristics as plant growth promoting rhizobacteria (PGPR). Both produced abscisic acid (ABA), indole‐3‐acetic acid (IAA) and the gibberellins A<sub>1</sub> and A<sub>3</sub> in chemically‐defined medium. They also colonized roots of in vitro grown <italic>Vitis vinifera</italic> cv. Malbec plants. As result of bacterization ABA levels in 45 days‐old in vitro plants were increased 76‐fold by <italic>B. licheniformis</italic> and 40‐fold by <italic>P. fluorescens</italic> as compared to controls. Both bacteria diminished plant water loss rate in correlation with increments of ABA. Twenty and 30 days post bacterization the plants incremented terpenes. The monoterpenes α‐pinene, terpinolene, 4‐carene, limonene, eucalyptol and lilac aldehyde A, and the sesquiterpenes α‐bergamotene, α‐farnesene, nerolidol and farnesol were assessed by gas chromatography‐electron impact mass spectrometry analysis. α‐Pinene and nerolidol were the most abundant (µg per g of tissue in plants bacterized with <italic>P. fluorescens</italic>). Only α‐pinene, eucalyptol and farnesol were identified at low concentration in non‐bacterized plants treated with ABA, while no terpenes were detected in controls. The results obtained along with others from literature suggest that <italic>B. licheniformis</italic> and <italic>P. fluorescens</italic> act as stress alleviators by inducing ABA synthesis so diminishing water losses. These bacteria also elicit synthesis of compounds of plant defense via an ABA independent mechanism.</p> </abstract>