Characterization of Schizothorax prenanti cgnrhII gene: fasting affects cgnrhII expression. Issue 2 (19th June 2014)

• Record Type: Journal Article
• Authors: Wang, T.; Yuan, D.; Zhou, C.; Lin, F.; Chen, H.; Wu, H.; Wei, R.; Xin, Z.; Liu, J.; Gao, Y.; Chen, D.; Yang, S.; Pu, Y.; Li, Z.
• Is Part Of: Journal of fish biology. Volume 85:Issue 2(2014)
• Page Start: 407
• Page End: 420
• Publication Date: 2014-06-19
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12430
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12430-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12430-para-0001">In this study, the role of chicken gonadotropin‐releasing hormone II (<italic>cgnrhII</italic>) in feeding regulation was investigated in <italic>Schizothorax prenanti</italic>. First, the full‐length <italic>S. prenanti cgnrhII</italic> cDNA consisted of 693 bp with an open reading frame of 261 bp encoding a protein of 86 amino acids. Next, <italic>cgnrhII</italic> was widely expressed in the central and peripheral tissues. Last, there were significant changes in <italic>cgnrhII</italic> mRNA expression in the fasted group compared to the fed group in the <italic>S. prenanti</italic> hypothalamus during 24 h fasting (<italic>P</italic> &lt; 0·05). Furthermore, the <italic>cgnrhII</italic> gene expression presented a significant decrease in the fasted group compared with the fed group (<italic>P</italic> &lt; 0·05) on days 3, 5 and 7, after re‐feeding, there was no significant changes in <italic>cgnrhII</italic> mRNA expression level between refed and fed group on day 9 (<italic>P</italic> &gt; 0·05). Thus, the results suggest that <italic>cGnRH</italic> II expression is influenced by fasting and the gene may be involved in feeding regulation in <italic>S. prenanti.</italic></p> </abstract>