Comparative studies of zebrafish Danio rerio lipoprotein lipase (lpl) and hepatic lipase (lipc) genes belonging to the lipase gene family: evolution and expression pattern. Issue 2 (6th June 2014)

• Record Type: Journal Article
• Authors: Feng, D.; Huang, Q. Y.; Liu, K.; Zhang, S. C.; Liu, Z. H.
• Is Part Of: Journal of fish biology. Volume 85:Issue 2(2014)
• Page Start: 329
• Page End: 342
• Publication Date: 2014-06-06
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12423
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12423-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12423-para-0001">In this study, bioinformatics analysis, tissue distribution and developmental expression pattern of lipoprotein lipase (<italic>lpl</italic>) and hepatic lipase (<italic>lipc</italic>) in zebrafish <italic>Danio rerio</italic> are reported. In adult <italic>D. rerio</italic>, <italic>lpl</italic> was highly expressed in liver. This is remarkably different from the tissue expression pattern of <italic>LPL</italic> in mammals, which is not detected in the adult liver. The expression of <italic>lipc</italic> was liver specific, which is consistent with that in mammals. During embryogenesis, <italic>lpl</italic> mRNA was increased gradually in concentration from 0·5 hpf (hour post fertilization) to 6 dpf (days post fertilization), but <italic>lipc</italic> was not expressed at the early stage of the embryo until 3 dpf. <italic>In situ</italic> hybridization further displayed the expression pattern of <italic>lpl</italic> mainly restricted to the head region including cells surrounding the mouth opening, branchial arches, pectoral fin and lateral line neuromast, whereas <italic>lipc</italic> was mainly restricted to the liver and part of head regions including lens. This lays a foundation for further investigation of <italic>lpl</italic> or <italic>lipc</italic> function and evolution in fishes.</p> </abstract>