Pro‐inflammatory cytokine release and cell growth inhibition in primary human oral cells after exposure to endodontic sealer. (28th January 2014)

• Record Type: Journal Article
• Authors: Diomede, F.; Caputi, S.; Merciaro, I.; Frisone, S.; D'Arcangelo, C.; Piattelli, A.; Trubiani, O.
• Is Part Of: International endontic journal. Volume 47:Number 9(2014:Sep.)
• Page Start: 864
• Page End: 872
• Publication Date: 2014-01-28
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12230
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej12230-abs-0001"> <title>Abstract</title> <sec id="iej12230-sec-0001" sec-type="section"> <title>Aim</title> <p>To assay the toxicity of the single‐methacrylate‐based sealer urethane dimethacrylate (UDMA) (EndoRez) in terms of cell growth and pro‐inflammatory cytokines release, in expanded <italic>ex vivo</italic> human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPDLSCs), human gingival fibroblasts (hGFs) and human osteoblasts (hOSTs).</p> </sec> <sec id="iej12230-sec-0002" sec-type="section"> <title>Methodology</title> <p>Dental pulp and periodontal ligament stem cells, osteoblasts and fibroblasts were derived from five young donors. After <italic>in vitro</italic> isolation, hDPSCs, hPDLSCs, hGFs and hOSTs were seeded to resin‐based sealers for 24, 48, 72 h up to 1 week. The morphological features and the cell growth and the release of pro‐inflammatory interleukin (IL)6, IL8, IL12 and tumour necrosis factor (TNF) α were analysed. Differences in cell growth and in interleukin secretion were analysed for statistical significance with two‐way <sc>anova</sc> tests for multiple comparisons.</p> </sec> <sec id="iej12230-sec-0003" sec-type="section"> <title>Results</title> <p>Exposure to endodontic sealer based on UDMA resulted in a 50% decrease in survival oral cells at 24 h of incubation. No evident morphological changes were present in cell cultures examined. After 48 h, 72 h and 1‐week culture time, a progressive cell growth was evident. A significant up‐regulation of IL6, IL8, IL12 and TNFα cytokines in cells in contact with the dental sealer compared to the control was observed.</p> </sec> <sec id="iej12230-sec-0004" sec-type="section"> <title>Conclusion</title> <p> <italic>In vitro</italic>, EndoRez interacted with primary human hDPSCs, hPDLSCs, hGFs and hOSTs causing damage to biological system evidenced through cell growth inhibition and up‐regulation of IL6, IL8, IL12 and TNFα proinflammatory mediators.</p> </sec> </abstract>