A New Tool Improves Diagnostic Test Performance for Transmission EM Evaluation of Axonemal Dynein Arms. (August 2014)

• Record Type: Journal Article
• Authors: Keith Funkhouser, W.; Niethammer, Marc; Carson, Johnny L.; Burns, Kimberlie A.; Knowles, Michael R.; Leigh, Margaret W.; Zariwala, Maimoona A.; Funkhouser, William K.
• Is Part Of: Ultrastructural pathology. Volume 38:Number 4(2014:Aug.)
• Page Start: 248
• Page End: 255
• Publication Date: 2014-08
• Subjects: Pathology, Cellular -- Periodicals; Ultrastructure (Biology) -- Periodicals; Diagnosis, Electron microscopic -- Periodicals; Microscopy, Electron; Pathology; 616.07582
• DOI: 10.3109/01913123.2013.815081
• Languages: English
• ISSNs: 0191-3123

Abstract

<abstract> <title>Abstract</title> <p>Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. The authors developed a novel image analysis tool to average the axonemal peripheral microtubular doublets, thereby increasing microtubular signal and reducing random background noise. In a randomized, double-blinded study that compared two experienced morphologists and three different diagnostic approaches, they found that use of this tool led to improvement in diagnostic TEM test performance.</p> </abstract>