In vitro and in vivo evaluation of a biphasic calcium phosphate scaffold coated with a native allogeneic extracellular matrix. (22nd June 2012)

• Record Type: Journal Article
• Authors: Zhou, Shifeng; Zhao, Wei; Liu, Xiaoqi; Liu, Guodan; Xi, Chunyang; Wang, Xintao; Yan, Jinglong
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 8:Number 8(2014:Aug.)
• Page Start: 620
• Page End: 628
• Publication Date: 2012-06-22
• Subjects: Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.1558
• Languages: English
• ISSNs: 1932-6254

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>This study evaluated the osteogenic properties of biphasic calcium phosphate (BCP) scaffolds coated with extracellular matrix (ECM) derived <italic>in vitro</italic> from allogeneic mesenchymal stem cells (MSCs). BCP/ECM and plain BCP scaffolds were seeded with MSCs from F344 rats and cultured in osteoinductive medium. At 1, 7, 14 and 21 days post‐seeding, assessments were made of cellularity, alkaline phosphatase (ALP) activity and RNA expression of osteocalcin, bone sialoprotein and osteopontin. MSCs seeded on BCP/ECM scaffolds exhibited significantly higher cellularity, ALP activity and transcript levels for the three genes examined. For the <italic>in vivo</italic> study, BCP/ECM and BCP scaffolds with and without MSCs were implanted subcutaneously into F344 rats. After four weeks of implantation, the extent of new bone formation and tissue response were examined by histology and histomorphometry; histological evidence showed that the seeded cell scaffolds induced new bone formation at the ectopic site and a higher average ratio of bone in the cell‐seeded BCP/ECM scaffold group. Results suggest that modification of the BCP scaffold with <italic>an in vitro</italic> generated allogeneic ECM can effectively enhance osteogenic properties <italic>in vitro</italic> and <italic>in vivo</italic>. Copyright © 2012 John Wiley &amp; Sons, Ltd.</p> </abstract>