Enzymatic hydrolysis of p‐nitrophenyl butyrate in CO2‐based micelle systems. Issue 9 (11th November 2013)

• Record Type: Journal Article
• Authors: Shang, Wenting; Zhang, Xiaogang; Yang, Xiaoxi; Zhang, Shuang
• Is Part Of: Journal of chemical technology & biotechnology. Volume 89:Issue 9(2014:Sep.)
• Page Start: 1324
• Page End: 1329
• Publication Date: 2013-11-11
• Subjects: Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.4246
• Languages: English
• ISSNs: 0268-2575

Abstract

<abstract abstract-type="main" id="jctb4246-abs-0001"> <title>Abstract</title> <sec id="jctb4246-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="jctb4246-para-0001"> <bold>In this work, CO<sub>2</sub>‐based micelles with TMN series surfactants were used as reactive medium. Hydrolysis of <italic>p</italic>‐nitrophenyl butyrate catalyzed by porcine pancreas was used as a model reaction. The reactive behavior was used to optimize the reaction temperature, pH, pressure, enzyme concentration and the added water.</bold> </p> </sec> <sec id="jctb4246-sec-0002" sec-type="section"> <title>RESULTS AND DISCUSSION</title> <p id="jctb4246-para-0002"> <bold>Generally, the reaction parameters depended on the types of substrates and enzymes. For the hydrolysis of <italic>p</italic>‐nitrophenyl butyrate in CO<sub>2</sub>‐based micelles, the reactive yield reached a high level at temperature 303 K, system pressure 15 MPa, pH 6.8, water to surfactant ratio <italic>W</italic><sub>0</sub> 15, and enzyme concentration 1 g mL<sup>‐1</sup>. In addition, the system reactivity was highest when TMN‐6 surfactant was used.</bold> </p> </sec> <sec id="jctb4246-sec-0003" sec-type="section"> <title>CONCLUSIONS</title> <p id="jctb4246-para-0003"> <bold>The activity of lipase‐catalyzed hydrolysis in CO<sub>2</sub>‐based micelle was increased significantly compared with that obtained in cyclohexane oil micelle. The combination of enzyme with supercritical CO<sub>2</sub> represented a promising 'green' reaction system for bioconversions. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>