Dyeing and antibacterial activation with methylene blue of a cyclodextrin modified polyester vascular graft. Issue 9 (1st October 2013)

• Record Type: Journal Article
• Authors: Kacem, I.; Laurent, T.; Blanchemain, N.; Neut, C.; Chai, F.; Haulon, S.; Hildebrand, H. F.; Martel, B.
• Is Part Of: Journal of biomedical materials research. Volume 102:Issue 9(2014)
• Page Start: 2942
• Page End: 2951
• Publication Date: 2013-10-01
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.34965
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The aim of this study was to develop an antiseptic and blue dyed polyester (PET) vascular graft in order to reach two distinct properties: (i) the prevention of postoperative infections, (ii) the improvement of the graft compatibility with the coelioscopy surgical technique. This work consisted of dyeing a vascular prosthesis with methylene blue (MB) which is known as a cationic dye with antiseptic properties. Therefore, the functionalization of the PET fibers of the prosthesis with a cyclodextrin–citric acid polymer (PolyCD) was achieved in order to improve its sorption capacity. The NMR experiments demonstrated that a 1:2 complex occurred between hydroxypropyl β‐cyclodextrin (HP‐βCD) and MB. Kinetic and sorption isotherm studies showed that an impregnation of the polyCD modified prosthesis (PET‐CD) in a 1 g L⁻¹ of MB solution for 150 min was sufficient to reach the saturation of the device. Results proved that the adsorption mechanism followed the Langmuir model and a maximum of 20 mg g⁻¹ of MB on the graft. A sustained release of MB in batch tests was observed in PBS and <italic>in vitro</italic> microbiological assays displayed a prolongation of the bactericidal effect of PET‐CD whose extent varied with the amount of MB preliminarily adsorbed onto the PET‐CD. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2942–2951, 2014.</p> </abstract>