A new, bioactive, antibacterial‐eluting, composite graft for infection‐free wound healing. Issue 4 (July 2014)

• Record Type: Journal Article
• Authors: Mittal, Anupama; Kumar, Neeraj
• Is Part Of: Wound repair and regeneration. Volume 22:Issue 4(2014)
• Page Start: 527
• Page End: 536
• Publication Date: 2014-07
• Subjects: Wound healing -- Periodicals; Regeneration (Biology) -- Periodicals; 617.14
• DOI: 10.1111/wrr.12194
• Languages: English
• ISSNs: 1067-1927

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The current work focuses on the in vivo performance of a newly developed injectable composite graft in infected full‐thickness wounds. The composite graft was composed of bioactive porous Poly dl‐lactide‐co‐glycolide scaffolds, antibiotic gentamicin, and crosslinked gelatin as carrier gel. Treated infected wounds exhibited a faster wound closure, rapid weight gain, lower neutrophil count, higher breaking strength, and 100 times lesser microbial count (10² colony forming units/g in infected treated vs. 10⁴ colony forming units/g in infected control group) in comparison with infected control group 28 days post treatment. During healing, collagen production was more in the treated groups at day 7 than controls and thereafter gradually reduced to normal levels. Histology revealed a mature scar tissue formation, fibroblast proliferation, epidermal resurfacing, and collagen deposition in reticular alignment similar to normal healthy skin in treated wounds. Further, the plasma concentration of gentamicin was 35–45 μg/mL during the initial 12 hours and reduced to 1 μg/mL in 24 hours, which indicated safe levels of the antibiotic drug during healing. These results clearly indicate a faster, infection‐free, and safe after treatment with the developed graft.</p> </abstract>