Detection and Molecular Variability of Turnip mosaic virus (TuMV) in Shaanxi, China. Issue 7 (20th December 2013)

• Record Type: Journal Article
• Authors: Zhao, Lei; Feng, Chaohong; Hao, Xingan; Wang, Renrui; Hu, Lingyun; Wang, Qiaochun; Wu, Yunfeng
• Is Part Of: Journal of phytopathology. Volume 162:Issue 7/8(2014)
• Page Start: 519
• Page End: 522
• Publication Date: 2013-12-20
• Subjects: Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12207
• Languages: English
• ISSNs: 0931-1785

Abstract

<abstract abstract-type="main" id="jph12207-abs-0001"> <title>Abstract</title> <p> <italic>Turnip mosaic virus</italic> (TuMV) is one of the most devastating threats to oilseed rape by causing serious crop losses. A total of 86 leaf samples of oilseed rape from eight different locations in Shaanxi, China, were tested by RT‐PCR for TuMV; the results revealed an infection level of 43% by TuMV. The complete coat protein (CP) gene of 32 TuMV isolates was cloned and sequenced. Analysis of the CP gene with sequences from the database allowed the genetic classification of 170 TuMV isolates or sequences. Four genetic clusters were obtained: MB (mostly <italic>Brassica</italic> isolates), MR (mostly <italic>Radish</italic> isolates), IBR (mostly Intermediate between <italic>Brassica</italic> and <italic>Radish</italic> clusters) and OBR (mostly outside <italic>Brassica</italic> and <italic>Radish</italic> clusters). All subgroups were slightly related to the hosts, but unrelated to geographical origins. Most of Shaanxi TuMV isolates were on separate branches, compared with the 138 known isolates originating from other parts of the world. Our results help provide a better understanding of the genetic diversity of TuMV isolates infecting oilseed rape in Shaanxi, China.</p> </abstract>