Antimicrobial activity of short‐ and medium‐term applications of polyhexamethylene biguanide, chlorhexidine digluconate and calcium hydroxide in infected immature bovine teeth in vitro. Issue 4 (2nd October 2013)

• Record Type: Journal Article
• Authors: Zaugg, Lucia K.; Zitzmann, Nicola U.; Hauser‐Gerspach, Irmgard; Waltimo, Tuomas; Weiger, Roland; Krastl, Gabriel
• Is Part Of: Dental traumatology. Volume 30:Issue 4(2014:Aug.)
• Page Start: 326
• Page End: 331
• Publication Date: 2013-10-02
• Subjects: Teeth -- Wounds and injuries -- Periodicals; Dentistry, Operative -- Periodicals; Traumatology -- Periodicals; Endodontics -- Periodicals; 617.6
• DOI: 10.1111/edt.12077
• Languages: English
• ISSNs: 1600-4469

Abstract

<abstract abstract-type="main" id="edt12077-abs-0001"> <title>Abstract</title> <sec id="edt12077-sec-0001" sec-type="section"> <title>Aim</title> <p>To compare the antimicrobial activity of polyhexamethylene biguanide (Prontosan wound gel, Pr) and chlorhexidine digluconate (CHX) after short‐ and medium‐term application with the disinfection ability of calcium hydroxide (Ca) in a model using immature bovine teeth.</p> </sec> <sec id="edt12077-sec-0002" sec-type="section"> <title>Methods</title> <p>Sixty immature bovine roots were infected with <italic>Enterococcus faecalis</italic> and randomly assigned to six groups (<italic>n </italic>= 10). Disinfectants were applied into the root canal for 10 min (CHX‐10 min and Pr‐10 min) or 7 days (CHX‐7d, Pr‐7d and Ca‐7d<sup>g</sup>). In the negative control group (Co‐n), no disinfectant was used. Dentine samples were collected, and the total count of bacteria and colony‐forming units were determined. The log<sub>10</sub>‐transformed Colony‐forming units (CFU) data were analysed using a Kruskal–Wallis test with <italic>post hoc</italic> Wilcoxon multiple‐comparison tests.</p> </sec> <sec id="edt12077-sec-0003" sec-type="section"> <title>Results</title> <p>The application of disinfectants led to a significant reduction in CFUs in all groups compared with group Co‐n. When compared to Ca‐7d<sup>g</sup>, CHX‐7d (<italic>P </italic>=<italic> </italic>0.290), CHX‐10 min (<italic>P </italic>=<italic> </italic>0.963) and Pr‐7d (<italic>P </italic>=<italic> </italic>0.095) revealed no significant differences. Pr‐10 min had a significantly higher CFU value than Ca‐7d<sup>g</sup> (<italic>P </italic>=<italic> </italic>0.0004), CHX‐10 min (<italic>P </italic>=<italic> </italic>0.0009) and Pr‐7d (<italic>P </italic>=<italic> </italic>0.0006).</p> </sec> <sec id="edt12077-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Within the limitations of this study, sufficient antimicrobial effect may be reached by a short‐term application of CHX. For the application of 1% Prontosan wound gel, a medium‐term use (7 day) is required, while short‐term use (10 min) is less effective.</p> </sec> </abstract>