In vivo imaging in an ABSL‐3 regional biocontainment laboratory. Issue 2 (18th June 2014)

• Record Type: Journal Article
• Authors: Scanga, Charles A.; Lopresti, Brian J.; Tomko, Jaime; Frye, Lonnie J.; Coleman, Teresa M.; Fillmore, Daniel; Carney, Jonathan P.; Lin, Philana L.; Flynn, JoAnne L.; Gardner, Christina L.; Sun, Chengqun; Klimstra, William B.; Ryman, Kate D.; Reed, Douglas S.; Fisher, Daniel J.; Cole, Kelly S.
• Is Part Of: Pathogens and disease. Volume 71:Issue 2(2014:Jul.)
• Page Start: 205
• Page End: 210
• Publication Date: 2014-06-18
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12186
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12186-abs-0001"> <title>Abstract</title> <p>The Regional Biocontainment Laboratory (RBL) at the University of Pittsburgh is a state‐of‐the‐art ABSL‐3 facility that supports research on highly pathogenic viruses and bacteria. Recent advances in radiologic imaging provide several noninvasive, <italic>in vivo</italic> imaging modalities that can be used to longitudinally monitor animals following experimental infection or vaccination. The University of Pittsburgh RBL provides digital radiography, bioluminescence imaging, and PET/CT. Operating these platforms in an ABSL‐3 poses unique challenges. This review will discuss the development and refinement of these imaging platforms in high containment, emphasizing specific challenges and how they were overcome.</p> </abstract>