Redox‐Based Assay for Assessment of Biological Impact of Plasma Treatment. Issue 7 (22nd April 2014)

• Record Type: Journal Article
• Authors: Wende, Kristian; Reuter, Stephan; von Woedtke, Thomas; Weltmann, Klaus‐Dieter; Masur, Kai
• Is Part Of: Plasma processes and polymers. Volume 11:Issue 7(2014:Jul.)
• Page Start: 655
• Page End: 663
• Publication Date: 2014-04-22
• Subjects: Plasma polymerization -- Periodicals; Plasma-enhanced chemical vapor deposition -- Periodicals; Plasma chemistry -- Periodicals
• DOI: 10.1002/ppap.201300172
• Languages: English
• ISSNs: 1612-8850

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="ppap201300172-sec-0001" sec-type="section"> <p>Commonly, non‐thermal plasma sources for medical applications are compared by the quantity of reactive components they produce. Here, a different perspective is chosen: A cell viability assay, which can act as standard procedure to compare plasma sources by the biological effectiveness is proposed. This assay can easily be performed with a minimum of technical equipment. It allows the estimation of the biological impact of a plasma source. For maximum flexibility, an indirect plasma treatment procedure is combined with a dilution method. The applicability of the assay is demonstrated by assessing the impact of different non‐thermal plasma sources (kINPen &amp; kINPen med, µAPPJ, water discharge, DBE) and the use of a series of human cell lines and their response to a kINPen treatment (HaCaT, MV3, Jurkat, THP‐1, MRC‐5).<inline-graphic xlink:href="ark:/27927/pghsf675q2" content-type="ppap201300172-gra-0001" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></p> </sec> </abstract>