Skin‐derived precursor schwann cell myelination capacity in focal tibial demyelination. Issue 2 (15th May 2014)

• Record Type: Journal Article
• Authors: Grochmal, Joey; Dhaliwal, Sundeep; Stys, Peter K.; van Minnen, Jan; Midha, Rajiv
• Is Part Of: Muscle & nerve. Volume 50:Issue 2(2014:Aug.)
• Page Start: 262
• Page End: 272
• Publication Date: 2014-05-15
• Subjects: Neuromuscular diseases -- Periodicals; Muscles -- Periodicals; Nerves -- Periodicals; 616.74
• DOI: 10.1002/mus.24136
• Languages: English
• ISSNs: 0148-639X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p> <italic>Introduction</italic>: Skin‐derived precursor cells (SKPs) are neural crest progenitor cells that can attain a Schwann cell–like phenotype through <italic>in vitro</italic> techniques (SKP‐SCs). We hypothesized that SKP‐SCs could produce mature myelin and, in doing so, facilitate the recovery of a focal demyelination injury. <italic>Methods</italic>: We unilaterally injected DiI‐labeled, green fluorescent protein (GFP)‐producing SKP‐SCs into the tibial nerves of 10 adult Lewis rats (with contralateral media control), 9 days after bilateral doxorubicin injury (0.38 μg). Tibial compound motor action potentials (CMAPs) were followed for 57 days. A separate morphometric cohort also included a Schwann cell injection group. <italic>Results</italic>: SKP‐injected nerves recovered fastest in terms of electrophysiology and morphometry. SKP‐SCs formed morphologically mature myelin, accounting for 15.3 ± 5.3% of the total myelin in SKP‐SC–injected nerves. <italic>Conclusions</italic>: SKP‐SCs are robustly capable of myelination. They improve the recovery of a focal tibial nerve demyelination model by myelinating a measured percentage of axons. <italic>Muscle Nerve</italic> 50:262–272, 2014</p> </abstract>