One‐step strategy for the synthesis of a derivatized cyclodextrin‐based monolithic column. Issue 14 (30th May 2014)

• Record Type: Journal Article
• Authors: Guo, Jialiang; Zhang, Qiaoxuan; Yao, Zhe; Zhao, Xianglong; Ran, Danni; Crommen, Jacques; Jiang, Zhengjin
• Is Part Of: Journal of separation science. Volume 37:Issue 14(2014:Jul.)
• Page Start: 1720
• Page End: 1727
• Publication Date: 2014-05-30
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201400312
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Derivatized β‐cyclodextrin (β‐CD) functionalized monolithic columns were prepared by a "one‐step" strategy using click chemistry. First, the intended derivatized β‐CD monomers were synthesized by a click reaction between propargyl methacrylate and mono‐6‐azido‐β‐CD and then sulfonation or methylation was carried out. Finally, monolithic columns were prepared through a one‐step <italic>in situ</italic> copolymerization of the derivatized β‐CD monomer and ethylene glycol dimethacrylate. The sulfated β‐CD‐based monolith was successfully applied to the hydrophilic interaction liquid chromatography separation of nucleosides and small peptides, while the methylated β‐CD‐functionalized monolith was useful for the separation of nonpolar compounds and drug enantiomers in capillary reversed‐phase liquid chromatography. The structures of the monomers were characterized by Fourier transform infrared spectroscopy and mass spectrometry. The physicochemical properties and column performance of monoliths were evaluated by scanning electron microscopy and micro high performance liquid chromatography. This strategy has considerable prospects for the preparation of other derivatized CD‐functionalized methacrylate monoliths.</p> </abstract>