MALDI‐MS analysis and imaging of small molecule metabolites with 1, 5‐diaminonaphthalene (DAN). (8th July 2014)

• Record Type: Journal Article
• Authors: Korte, Andrew R.; Lee, Young Jin
• Is Part Of: Journal of mass spectrometry. Volume 49:Number 8(2014:Aug.)
• Page Start: 737
• Page End: 741
• Publication Date: 2014-07-08
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.3400
• Languages: English
• ISSNs: 1076-5174

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>1, 5‐diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix‐assisted laser desorption ionization‐mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metabolite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW &lt; ~400 Da; however, 9‐aminoacridine (9‐AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative profile of a natural phospholipid mixture than 9‐AA. Finally, DAN and 9‐AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.</p> </abstract>