Characterization and Functional Analysis of Voltage‐Dependent Anion Channel 1 (VDAC1) from Orange‐Spotted Grouper (Epinephelus coioides). Issue 7 (30th May 2014)

• Record Type: Journal Article
• Authors: Shi, Yan; Zhao, Zhe; Hong, Xiaoyou; Chen, Kunci; Zhu, Xinping
• Is Part Of: Journal of biochemical and molecular toxicology. Volume 28:Issue 7(2014:Jul.)
• Page Start: 292
• Page End: 301
• Publication Date: 2014-05-30
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Toxicology -- Periodicals; 574
• DOI: 10.1002/jbt.21565
• Languages: English
• ISSNs: 1095-6670

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>The voltage‐dependent anion channel (VDAC) is a highly conserved integral protein of mitochondria in different eukaryotic species. It forms a selective channel in the mitochondrial outer membrane that serves as the controlled pathway for small metabolites and ions. In this study, a <italic>VDAC</italic> gene, <italic>EcVDAC1</italic>, was isolated from orange‐spotted grouper (<italic>Epinephelus coioides</italic>). The <italic>EcVDAC1</italic> exhibits ubiquitous expression in various tissues of orange‐spotted grouper and is upregulated in liver, gill, and spleen after stimulation with lipopolysaccharides (LPS). Subcellular localization analysis shows that the <italic>Ec</italic>VDAC1 protein colocalized with the mitochondria. A caspase‐3 assay demonstrates that overexpression of the <italic>EcVDAC1</italic> induced apoptotic cell death in fathead minnow cells. The data presented in this study provide new information regarding the relationship between LPS and the <italic>EcVDAC1</italic> gene, suggesting that the fish <italic>VDAC1</italic> gene may play an important role in antibacterial immune response.</p> </abstract>