Osteosarcoma Cells Enhance Angiogenesis Visualized by Color‐Coded Imaging in the In Vivo Gelfoam® Assay. Issue 9 (September 2014)

• Record Type: Journal Article
• Authors: Uehara, Fuminari; Tome, Yasunori; Miwa, Shinji; Hiroshima, Yukihiko; Yano, Shuya; Yamamoto, Mako; Mii, Sumiyuki; Maehara, Hiroki; Bouvet, Michael; Kanaya, Fuminori; Hoffman, Robert M.
• Is Part Of: Journal of cellular biochemistry. Volume 115:Issue 9(2014:Sep.)
• Page Start: 1490
• Page End: 1494
• Publication Date: 2014-09
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.24799
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb24799-sec-0001" sec-type="section"> <p>We previously described a color‐coded imaging model that can quantify the length of nascent blood vessels using Gelfoam® implanted in nestin‐driven green fluorescent protein (ND‐GFP) nude mice. In ND‐GFP mice, nascent blood vessels are labeled with GFP. We report here that osteosarcoma cells promote angiogenesis in the Gelfoam® angiogenesis assay in ND‐GFP mice. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic ND‐GFP nude mice. Seven days after transplantation of Gelfoam®, skin flaps were made and human 143B osteosarcoma cells expressing green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in cytoplasm were injected into the transplanted Gelfoam®. The control‐group mice had only implanted Gelfoam®. Skin flaps were made at days 14, 21, and 28 after transplantation of the Gelfoam® to allow imaging of vascularization in the Gelfoam® using a variable‐magnification small animal imaging system and confocal fluorescence microscopy. ND‐GFP expressing nascent blood vessels penetrated and spread into the Gelfoam® in a time‐dependent manner in both control and osteosarcoma‐implanted mice. ND‐GFP expressing blood vessels in the Gelfoam® of the osteosarcoma‐implanted mice were associated with the cancer cells and larger and longer than in the Gelfoam®‐only implanted mice (<italic>P</italic> < 0.01). The results presented in this report demonstrate strong angiogenesis induction by osteosarcoma cells and suggest this process is a potential therapeutic target for this disease. J. Cell. Biochem. 115: 1490–1494, 2014. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>