GuA6DT, a Regiospecific Prenyltransferase from Glycyrrhiza uralensis, Catalyzes the 6‐Prenylation of Flavones. Issue 11 (8th July 2014)

• Record Type: Journal Article
• Authors: Li, Jianhua; Chen, Ridao; Wang, Ruishan; Liu, Xiao; Xie, Dan; Zou, Jianhua; Dai, Jungui
• Is Part Of: Chembiochem. Volume 15:Issue 11(2014)
• Page Start: 1673
• Page End: 1681
• Publication Date: 2014-07-08
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201402160
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>GuA6DT, a flavonoid prenyltransferase, was identified from <italic>Glycyrrhiza uralensis</italic>, and it was found that this enzyme regiospecifically transfers a dimethylallyl moiety to apigenin at the C‐6 position. A further substrate specificity investigation indicated that the existence of hydroxyls at both the C‐5 and C‐7 positions of the flavone skeleton is critical for the prenylation. However, substitutions on the B‐ring had negligible influence on the prenylation. A comparison of <italic>GuA6DT</italic> expression in different organs revealed that mRNA is mainly expressed in the aerial parts. Moreover, the <italic>GuA6DT</italic> mRNA was found to be regulated at the transcriptional level, because methyl jasmonate induced upregulation in cultured cells. GuA6DT is the first identified flavone prenyltransferase to exhibit strict substrate specificity and regiospecificity.</p> </abstract>