Apoptosis‐Like Events and In Vitro Fertilization Capacity of Sex‐Sorted Bovine Sperm. Issue 4 (4th June 2014)

• Record Type: Journal Article
• Authors: Zhao, X‐M; Ren, J‐J; Zhao, S‐J; Cui, L‐S; Hao, H‐S; Wang, H‐Y; Du, W‐H; Qin, T; Liu, Y; Wang, D; Zhu, H‐B
• Is Part Of: Reproduction in domestic animals. Volume 49:Issue 4(2014)
• Page Start: 543
• Page End: 549
• Publication Date: 2014-06-04
• Subjects: Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/rda.12305
• Languages: English
• ISSNs: 0936-6768

Abstract

<abstract abstract-type="main" id="rda12305-abs-0001"> <title>Contents</title> <p>This study utilized three staining assays (Annexin V, mitochondrial membrane potential (JC‐1) and TUNEL) for flow cytometric analysis of apoptosis in sex‐sorted sperm from four different bulls (A, B, C and D). Correlations between sperm quality and IVF efficiency were then assessed to determine which assay provided the best prediction of IVF efficiency. The results of the Annexin V assays, as well as measures of viable sperm, early apoptosis, necrotic sperm and mitochondrial membrane potential (∆<italic>ψ</italic>m) showed that the sex‐sorted sperm collected from bull A significantly differed from those of the other three bulls (p<italic> < </italic>0.05). In addition, the levels of DNA fragmentation in sex‐sorted sperm from bull A were significantly lower than those from bulls B and C (p<italic> < </italic>0.05). The percentage of cells reaching the cleavage and blastocyst stages in sex‐sorted sperm from bull A were significantly greater than those from the other bulls (p<italic> < </italic>0.05). A significant positive correlation was observed between viable sperm and the percentage of cells at the cleavage or blastocyst stages (p<italic> < </italic>0.05). In contrast, a negative correlation was found between early apoptotic sperm and the percentage of cells at the cleavage or blastocyst stages (p<italic> < </italic>0.05). In conclusion, these results indicated that the Annexin V assay was the most reliable technique for the prediction of the IVF success of sex‐sorted bovine sperm.</p> </abstract>