Distinct B‐cell populations contribute to vaccine antigen‐specific antibody production in a transgenic mouse model. Issue 4 (August 2014)

• Record Type: Journal Article
• Authors: O, Eunju; Ko, Eun‐Ju; Kim, Min‐Chul; Lee, Young‐Tae; Song, Jae‐Min; Kwon, Young‐Man; Compans, Richard W.; Kang, Sang‐Moo
• Is Part Of: Immunology. Volume 142:Issue 4(2014:Aug.)
• Page Start: 624
• Page End: 635
• Publication Date: 2014-08
• Subjects: Immunology -- Periodicals
• DOI: 10.1111/imm.12287
• Languages: English
• ISSNs: 0019-2805

Abstract

<abstract abstract-type="main" id="imm12287-abs-0001"> <title>Summary</title> <p>The generation of memory B cells by vaccination plays a critical role in maintaining antigen‐specific antibodies and producing antibody responses upon re‐exposure to a pathogen. B‐cell populations contributing to antibody production and protection by vaccination remain poorly defined. We used influenza virus‐like particle (VLP) vaccine in a transgenic mouse model that would identify germinal centre‐derived memory B cells with the expression of yellow fluorescent protein (YFP<sup>+</sup> cells). Immunization with influenza VLP vaccine did not induce significant increases in YFP<sup>+</sup> cells although vaccine antigen‐specific antibodies in sera were found to confer protection against a lethal dose of influenza A virus (A/PR8). In addition, CD43<sup>+</sup> B220<sup>−</sup> populations with low YFP<sup>+</sup> cells mainly contributed to the production of vaccine antigen‐specific IgG isotype‐switched antibodies whereas CD43<sup>−</sup> B220<sup>+</sup> populations with high YFP<sup>+</sup> cells were able to produce vaccine antigen‐specific IgM antibodies. Challenge infection of immunized transgenic mice with live influenza A virus resulted in significant increases in YFP<sup>+</sup> cells in the B220<sup>−</sup> populations of spleen and bone marrow cells. These results suggest that CD43<sup>+</sup> B220<sup>−</sup> B cells generated by vaccination are important for producing influenza vaccine antigen‐specific antibodies and conferring protection.</p> </abstract>