Expression of IFN‐γ, IL‐4, and IL‐17 in cutaneous schistosomal granuloma. (6th March 2014)

• Record Type: Journal Article
• Authors: Attia, Sameh K.; Moftah, Noha H.; Abdel‐Azim, Eman S.
• Is Part Of: International journal of dermatology. Volume 53:Number 8(2014:Aug.)
• Page Start: 991
• Page End: 998
• Publication Date: 2014-03-06
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/ijd.12464
• Languages: English
• ISSNs: 0011-9059

Abstract

<abstract abstract-type="main" id="ijd12464-abs-0001"> <title>Abstract</title> <sec id="ijd12464-sec-0001" sec-type="section"> <title>Objectives</title> <p>Cutaneous schistosomal granuloma (CSG) is a rare dermatological disease, the clinical and histopathological features of which are well defined. Although a panoramic picture of its immunopathogenesis in humans is not yet available, it is believed to be induced by T helper 1 (Th1), Th2, or Th17 cytokines in animals. This study evaluated the expression of different types of Th cytokines, including Th1 cytokine interferon‐<italic>γ</italic> (IFN‐<italic>γ</italic>), Th2 cytokine interleukin‐4 (IL‐4), and Th17 cytokine IL‐17, in human CSG.</p> </sec> <sec id="ijd12464-sec-0002" sec-type="section"> <title>Methods</title> <p>This study included nine patients with CSG. Dermatological examinations were conducted in all subjects. Skin biopsy specimens were stained with hematoxylin and eosin (H&E). Immunohistochemical examination was performed using three monoclonal anti‐human antibodies against IFN‐<italic>γ</italic>, IL‐4, and IL‐17 to evaluate Th1, Th2, and Th17 cytokines, respectively.</p> </sec> <sec id="ijd12464-sec-0003" sec-type="section"> <title>Results</title> <p>The most common site of CSG manifestation was the paraumbilical area, which was affected in 66.7% of patients. All lesional skin biopsy specimens revealed multiple dermal granulomas surrounding schistosomal eggs. Positive immunoreactivity for IFN‐<italic>γ</italic>, IL‐4, and IL‐17 was present in dermal inflammatory infiltrate in 88.9, 11.1, and 88.9% of subjects, respectively. There were statistically significant negative correlations between the duration of disease and both IFN‐<italic>γ</italic> and IL‐17 (<italic>P </italic>≤ 0.05), and a statistically significant positive correlation between IFN‐<italic>γ</italic> and IL‐17 (<italic>P </italic>≤ 0.05).</p> </sec> <sec id="ijd12464-sec-0004" sec-type="section"> <title>Conclusions</title> <p>This study suggests that CSG is formed by the action of both Th1 (IFN‐<italic>γ</italic>) and Th17 (IL‐17) cytokines, which have been shown to be directed against the schistosomal egg to induce a cell‐mediated immune response.</p> </sec> </abstract>