Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental × Qinchuan steers. Issue 3 (14th August 2014)

• Record Type: Journal Article
• Authors: Li, Xiaomin; Yang, You; Liu, Shimin; Yang, Jing; Chen, Cheng; Sun, Zhihong
• Is Part Of: British journal of nutrition. Volume 112:Issue 3(2014)
• Page Start: 347
• Page End: 357
• Publication Date: 2014-08-14
• Subjects: Nutrition -- Periodicals; 572.4
• DOI: 10.1017/S0007114514001032
• Languages: English
• ISSNs: 0007-1145

Abstract

<abstract abstract-type="normal"> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <p>Grape seed extract (GSE), a rich source of polyphenols, is reported to possess antioxidant, anti-inflammatory and immunomodulatory properties. The objective of the present study was to determine whether GSE could attenuate the heat stress-induced responses of jejunum epithelial cells (JEC) in cattle. The JEC of a steer (Simmental × Qinchuan) were exposed to heat stress for 2 h in the absence (0 μg/ml) or presence (10, 20, 40 and 80 μg/ml) of GSE in the culture medium. When cultured at 40°C, JEC supplemented with GSE exhibited increased glutathione peroxidase activity (<italic>P</italic>= 0·04), viability (<italic>P</italic>= 0·004), and mRNA expression of epidermal growth factor (<italic>EGF</italic>; <italic>P</italic>= 0·03) and EGF receptor (<italic>EGFR</italic>; <italic>P</italic>= 0·01). Under the same conditions, the cells exhibited decreased mRNA expression of <italic>IL-8</italic> (<italic>P</italic>= 0·01) and <italic>TNF-α</italic> (<italic>P</italic>= 0·03) and decreased protein concentrations of IL-1β (<italic>P</italic>= 0·02), Toll-like receptor 4 (TLR4; <italic>P</italic>= 0·04) and heat shock protein 70 (HSP70; <italic>P</italic>< 0·001). When cultured at 43°C, JEC supplemented with GSE exhibited increased catalase activity (<italic>P</italic>= 0·04), viability (<italic>P</italic>< 0·001), and mRNA expression of <italic>EGF</italic> (<italic>P</italic>< 0·001) and <italic>EGFR</italic> (<italic>P</italic>< 0·001) and decreased protein concentrations of IL-1β (<italic>P</italic>< 0·001), TLR4 (<italic>P</italic>= 0·03) and HSP70 (<italic>P</italic>< 0·001), as well as mRNA expression of <italic>IL-8</italic> (<italic>P</italic>< 0·001), <italic>TLR4</italic> (<italic>P</italic>= 0·002) and <italic>TNF-α</italic> (<italic>P</italic>< 0·001). Temperature × GSE concentration interactions were also observed for the concentrations of IL-1β (<italic>P</italic>< 0·001), IL-8 (<italic>P</italic>< 0·001), TNF-α (<italic>P</italic>= 0·01) and HSP70 (<italic>P</italic>= 0·04) and viability (<italic>P</italic>< 0·001) of JEC. The results of the present study indicate that GSE can attenuate the responses of JEC induced by heat stress within a certain range of temperatures.</p> </abstract>