Clinical significance of expression and epigenetic profiling of TUSC1 in gastric cancer. Issue 2 (2nd April 2014)

• Record Type: Journal Article
• Authors: Kanda, Mitsuro; Shimizu, Dai; Nomoto, Shuji; Hibino, Soki; Oya, Hisaharu; Takami, Hideki; Kobayashi, Daisuke; Yamada, Suguru; Inokawa, Yoshikuni; Tanaka, Chie; Fujii, Tsutomu; Sugimoto, Hiroyuki; Koike, Masahiko; Fujiwara, Michitaka; Kodera, Yasuhiro
• Is Part Of: Journal of surgical oncology. Volume 110:Issue 2(2014:Aug. 01)
• Page Start: 136
• Page End: 144
• Publication Date: 2014-04-02
• Subjects: Cancer -- Surgery -- Periodicals; Neoplasms -- Periodicals; 616
• DOI: 10.1002/jso.23614
• Languages: English
• ISSNs: 0022-4790

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jso23614-sec-0001" sec-type="section"> <title>Background and Objectives</title> <p>The prognosis of advanced gastric cancer (GC) remains dismal. The aim of this study was to identify a novel tumor suppressor gene (TSG) with repressed transcription by aberrant DNA methylation in GC.</p> </sec> <sec id="jso23614-sec-0002" sec-type="section"> <title>Methods</title> <p>The expression and methylation status of tumor suppressor candidate 1 (<italic>TUSC1</italic>) were evaluated in GC cell lines and 112 pairs of surgical specimens. <italic>TUSC1</italic> protein expression and distribution in GC tissue were determined by immunohistochemistry.</p> </sec> <sec id="jso23614-sec-0003" sec-type="section"> <title>Results</title> <p>The majority of GC cell lines (83%) and GC tissues (82%) showed downregulation of <italic>TUSC1</italic> mRNA compared with noncancerous tissues. No significant differences were found in <italic>TUSC1</italic> mRNA expression between three GC subtypes categorized by tumor locations and morphology. Reduced expression of <italic>TUSC1</italic> mRNA in GC tissues was significantly associated with advanced T stage, vessel invasion and lymph node metastasis, leading to poor prognosis. The expression patterns of <italic>TUSC1</italic> protein were confirmed to be consistent with those of <italic>TUSC1</italic> mRNA. Sixty‐three (57%) of 112 patients showed intragenic hypermethylation of <italic>TUSC1</italic> in GC tissues.</p> </sec> <sec id="jso23614-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Our results suggested that reduced expression of <italic>TUSC1</italic> mRNA was related to poor prognosis and <italic>TUSC1</italic> is a putative TSG that is suppressed through intragenic hypermethylation in GC. <italic>J. Surg. Oncol. 2014; 110:136–144</italic>. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>